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RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

ABSTRACT: RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

PROVIDER: PRJEB65973 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12037205.fastq.gz	Fastqsanger.gz
	ERR12037206.fastq.gz	Fastqsanger.gz
	ERR12037207.fastq.gz	Fastqsanger.gz
	ERR12037208.fastq.gz	Fastqsanger.gz
	ERR12037209.fastq.gz	Fastqsanger.gz

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RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

Project description:Analysis of the global variability of gene expression between the Eif6 WT control genotype (CTRL) and the Eif6 S235A mutated (SA) MEF lines. To analyze the effects over time of the lack of Eif6 phosphorylation, we transduced MEFs with tamoxifen-inducible Cre, so that treatment leads to excision of wild-type exons and the generation of cells bearing the S235A mutation. The analysis is done on three replicates per genotype.

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RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

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40S footprints of Eif6 WT and Eif6 S235A MEFs

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Ribo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

Project description:Ribo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

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Project description:Ribo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

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RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

Project description:RNA-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

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Ribo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

Project description:Analysis of global variability of translation between the Eif6 WT control genotype and the Eif6 S235A mutated MEF lines. Ribosome Protected Fragments (RPFs) were obtained followwing the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028).

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Ribo-seq of Eif6 WT and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)

Project description:Analysis of global variability of translation between the Eif6 WT control genotype (CTRL) and the Eif6 S235A (SA) mutated MEF lines. The analysis is done on three replicates per genotype. Ribosome Protected Fragments (RPFs) were obtained following the procedure reported in McGlincy NJ & Ingolia NT. Methods. 2017 (doi: 10.1016/j.ymeth.2017.05.028).

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Project description:Analysis of global variability of translation initiation between the Eif6 WT control genotype and the Eif6 S235A mutated MEF lines pre-treated with harringtonine. Ribosome Protected Fragments (RPFs) were obtained following the method reported in Ingolia NT et al., NatProtoc 2012. (doi:10.1038/nprot.2012.086)

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