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PySymPilo

ABSTRACT: Symbiodinium pilosum

PROVIDER: PRJEB66063 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	pySymPilo4.Algiphilus_sp_1.1.primary.fa.gz	Other
	pySymPilo4.Aquicoccus_porphyridii_1.1.primary.fa.gz	Other
	pySymPilo4.metagenome.1.primary.fa.gz	Other
	pySymPilo4.Qipengyuania_sp_1.1.primary.fa.gz	Other

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The host transcriptome remains unaltered during the establishment of coral-algal symbioses

Project description:Coral reefs are based on the symbiotic relationship between corals and photosynthetic dinoflagellates of the genus Symbiodinium. We followed gene expression of coral larvae of Acropora palmata and Montastraea faveolata after exposure to Symbiodinium strains that differed in their ability to establish symbioses. We show that the coral host transcriptome remains almost unchanged during infection by competent symbionts, but is massively altered by symbionts that fail to establish symbioses. Our data suggest that successful coral-algal symbioses depend mainly on the symbionts' ability to enter the host in a stealth manner rather than a more active response from the coral host. Acropora palmata Samples: Three biological replicates of pooled larvae from each species and condition (i.e. untreated control, inoculated with competent Symbiodinium strain, inoculated with incompetent Symbiodinium strain) for both time points were hybridized against a pooled reference. Pooled references were constructed by combining equal amounts of aRNA from all control samples from A. palmata. References were labeled with Cy3, samples with Cy5. Montastraea faveolata Samples: Three biological replicates of pooled larvae from each species and condition (i.e. untreated control, inoculated with competent Symbiodinium strain, inoculated with incompetent Symbiodinium strain) for both time points were hybridized against a pooled reference. Pooled references were constructed by combining equal amounts of aRNA from all control samples from M. faveolata. References were labeled with Cy3, samples with Cy5. Symbiodinium sp. CassKB8: competent strain Symbiodinium sp. EL1: incompetent strain Symbiodinium sp. Mf1.05b: competent strain

2010-06-20 | E-GEOD-14923 | biostudies-arrayexpress

Sex, Scavengers, and Chaperones: Transcriptome Secrets of Divergent Symbiodinium Thermal Tolerances

Project description:Using transcriptomics, we show that Symbiodinium acclimation to elevated temperature involves up-regulated expression of meiosis genes followed by up-regulated expression of numerous reactive oxygen species scavenging genes and molecular chaperone genes. Our study connects Symbiodinium transcriptional regulation with physiological heat stress responses as well as known bleaching responses of corals harboring these same Symbiodinium. By uncovering these critical links, we greatly advance understanding of the bleaching susceptibility of corals, which is a key process responsible for global coral reef health.

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De novo assembly and transcriptome analysis of Ocimum americanum var. pilosum under low temperature

Project description:One-month-old Ocimum americanum var. pilosum plants were treated with 4 ºC for 12 hours. Total RNAs were extracted from these chilling-treated plant leaves and untreated ones. Two seperate replicates of total RNA samples are from different individuals. The genomic DNA residual in each sample was removed by DNase I digestion.

2015-05-19 | GSE68980 | GEO

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