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Transcriptomic profiling reveals key early response genes during GDF6-mediated differentiation of human adipose-derived stem cells to nucleus pulposus cells

ABSTRACT: Transcriptomic profiling reveals key early response genes during GDF6-mediated differentiation of human adipose-derived stem cells to nucleus pulposus cells

PROVIDER: PRJEB67513 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12121703_1.fastq.gz	Fastqsanger.gz
	ERR12121703_2.fastq.gz	Fastqsanger.gz
	ERR12121704_1.fastq.gz	Fastqsanger.gz
	ERR12121704_2.fastq.gz	Fastqsanger.gz
	ERR12121705_1.fastq.gz	Fastqsanger.gz

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Transcriptomic profiling reveals key early response genes during GDF6-mediated differentiation of human adipose-derived stem cells to nucleus pulposus cells

Project description:Stem cell-based therapies show promise as a means of repairing the degenerate intervertebral disc, with growth factors often used alongside cells to help direct differentiation towards a nucleus pulposus (NP)-like phenotype. We previously demonstrated adipose-derived stem cell (ASCs) differentiation with GDF6 as optimal for generating NP-like cells through evaluating end-stage differentiation parameters. Here we conducted a time-resolved transcriptomic characterisation of ASCs response to GDF6 stimulation to understand the early drivers of differentiation to NP-like cells.

2025-03-26 | E-MTAB-13435 | biostudies-arrayexpress

Expression data from synovium, bone marrow and nucleus pulposus

Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed. Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.

2010-10-11 | E-GEOD-24612 | biostudies-arrayexpress

MicroRNAs expression profiling of human nucleus pulposus cells: control vs. degeneration

Project description:MicroRNAs expression profiling of human nucleus pulposus cells derived from patients with disc degeneration in comparison with those derived from patients with scoliosis as control. Two-condition experiment: control nucleus pulposus cells vs. degenerative nucleus pulposus cells. Biological replicates: 3 control, 3 degenerated, independently harvested. Four replicates per array. The supplementary file 'GSE19943_fold_pvalue.txt' contains fold-changes and p-values.

2012-01-01 | E-GEOD-19943 | biostudies-arrayexpress

Expression data from bovine nucleus pulposus interverteral disc cells

Project description:Assessment of the putative differential gene expression profiles in high osmolality-treated bovine nucleus pulposus intervertebral disc cells for a short (5 h) and a long (24 h) time period. Identification of novel genes up- or down-regulated as an early or a late response to hyperosmotic stress. A 5 and 24 h-hyperosmotic treatment of nucleus pulposus cells led to transcriptional changes in >100 and 200 genes, respectively. Nucleus pulposus intervertebral disc cells were exposed to hyperosmotic stress for 5 and 24 h before RNA extraction and transcriptomics analysis. Three biological replicates were tested for each condition. Selected genes found to be differentially expressed were validated by RT-qPCR. Functional experiments were performed in order to assess the role of specific proteins encoded by genes found to be up-regulated in the osmo-reguatory response of intervertebral disc cells.

2015-06-19 | E-GEOD-59485 | biostudies-arrayexpress

human nucleus pulposus cell LC-MS/MS

Project description:To investigate whether SUV39H2 binds to PPP1CA in nucleus pulposus cells

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Expression data from young and old nucleus pulposus stem cells

Project description:Nucleus pulposus stem cells is the endogenous stem cell in nucleus pulposus tissues(NPMSCs). The senescene of NPMSCs will cause its malfunction and further induce intervertebral disc degeneration.The expression of specific sets of genes will altered during aging. Thus, identify these key regulater genes is important. We used microarrays to detail the global programme of gene expression underlying NPMSCs aging and identified distinct classes of up and down-regulated genes during this process

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A transcriptome analysis of serum-starved nucleus pulposus cells

Project description:This study sought to elucidate the transcriptomic changes of nucleus pulposus cells in serum starvation. The findings will lead to a better understanding of quiescent nucleus pulposus cells and provide insights into disc degeneration at the molecular level.

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Expression data from synovium, bone marrow and nucleus pulposus

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Effect of recombinant Shh on gene expression of primary rat nucleus pulposus cells

Project description:In order to study the effect of recombinant Shh (rShh) on nucleus pulposus cells, primary nucleus pulposus cells from SD rats were obtained.Then, nucleus pulposus cells were treated with rShh at 200 ng/ml(dissolved in dmso) for 48h. Similarly, we treated nucleus pulposus cells with the same concentration of DMSO as the control group, and analyzed the data of 3 biological repeats of RNA-seq in each group for gene expression profile analysis.

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2023-10-24 | PXD042397 | Pride