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ScRNA-seq of pluripotent stem cell-derived human intestinal organoids grown in Epiregulin

ABSTRACT: scRNA-seq of pluripotent stem cell-derived human intestinal organoids grown in Epiregulin

PROVIDER: PRJEB67635 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12138138_1.fastq.gz	Fastqsanger.gz
	ERR12138138_2.fastq.gz	Fastqsanger.gz
	ERR12138139_1.fastq.gz	Fastqsanger.gz
	ERR12138139_2.fastq.gz	Fastqsanger.gz
	ERR12138140_1.fastq.gz	Fastqsanger.gz

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scRNA-seq of pluripotent stem cell-derived human intestinal organoids grown in Epiregulin

Project description:Here, we used single-cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell-derived human intestinal organoids (HIOs) grown in media comprised of minigut media + varying concentrations of Epiregulin (EREG) 1 ng/ml, 10 ng/ml, 100 ng/ml after 28 days of in vitro growth.

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Project description:Here, we used single cell RNA-sequencing (scRNA-seq) to profile pluripotent stem cell derived human definitive endoderm and intestinal organoids (HIOs) at several timepoints of in vitro growth (7, 14, and 28 days) and after in vivo growth beneath the kidney capsule of a murine host (4 and 8 wks post-transplant). Additionally, we profiled HIOs grown in a non-adhesive alginate hydrogel and also CDX2 knockout HIOs. In order to benchmark the organoid cultures, we used scRNA-seq to profile primary human fetal esophagus (14.3 pcw, 16.7 pcw), stomach (6.7, 14.3, and 16.7 pcw), liver (14.4 pcw), small intestine ( 11.4 and 14.4 pcw) and colon (11.4, 14.4, and 18.9 pcw). Diverse cell lineages were captured across all tissues profiled, including: epithelium, mesenchyme, neurons, endothelium, and immune lineages.

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Project description:scRNA-seq of pluripotent stem cell derived human intestinal organoids grown in an alginate matrix

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Project description:scRNA-seq of pluripotent stem cell derived human intestinal organoids

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