Ixodes ricinus (tick)
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB67792 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:There has been an emergence and expansion of tick-borne diseases in Europe, Asia and North America in recent years, including Lyme disease, tick-borne encephalitis, and human anaplasmosis. The primary tick vectors implicated are hard ticks of the Ixodes genera. Although much is known about the host response to these bacterial and viral pathogens, there is limited knowledge of the cellular responses to infection within the tick vector. The bacterium Anaplasma phagocytophilum (A. phagocytophilum), is able to bypass apoptotic processes in ticks, enabling infection to proceed. However, the tick cellular responses to infection with the flaviviruses tick-borne encephalitis virus (TBEV) and louping ill virus (LIV), which cause tick-borne encephalitis and louping ill respectively, are less clear. Infection of an Ixodes ricinus (I. ricinus) tick cell line with the viruses LIV and TBEV, and the bacterium A. phagocytophilum, identified activation of common and distinct cellular pathways. In particular, commonly-upregulated genes included those that modulate apoptotic pathways (HSP70), putative anti-pathogen genes (FKBP and XBL1), and genes that influence the tick innate immune response, including selective activation of toll genes. These data provide an insight into potentially key genes involved in the tick cellular response to viral or bacterial infection.
2017-01-01 | GSE85300 | GEO
Project description:Tick bites often predispose to meat allergy. The mechanisms involved in this process is not fully understood. Here we investigated the changes in the gut induced by Ixodes ricinus bites.
2024-11-21 | GSE247064 | GEO
Project description:Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation.
2021-09-09 | PXD021370 | Pride
Project description:Tick bites often promote local allergic reactions in the skin. The mechanisms involved in this process is not fully understood. Here we investigated the local changes in the skin induced by Ixodes ricinus bites.
2024-11-21 | GSE247060 | GEO
Project description:We report differential gene expression with tissue-specific signatures in tick cell lines infected with Anaplasma phagocytophilum - transcriptional response to infection of I. scapularis ISE6 cells resembled that of tick hemocytes while the response in I. ricinus IRE/CTVM20 cells resembles that of tick midguts.
2017-01-01 | GSE76906 | GEO
Project description:Ticks are hematophagous ectoparasites that impact human and animal health worldwide. Tick saliva contains multiple biomolecules capable of disrupting host immunity, occasionally causing emergent tick-borne allergies like the alpha-Gal syndrome (AGS). Current results highlight a knowledge gap in characterizing tick salivary biomolecules, without alpha-Gal modifications, that influence host response to alpha-Gal and may be implicated in the AGS. This study aimed to functionally characterize biomolecules antigen p23 (A0A0K8RKR7) and metalloprotease (A0A0K8RCY8) based on their role in Ixodes ricinus tick feeding and reproduction, determined after gene knockdown by RNA interference and further transcriptomics analysis. Silencing the expression of antigen p23 and metalloprotease did not result in any significant differences in tick engorgement and egg batch weights compared to the GFP control group. Transcriptomics analysis revealed a total of 438 differentially expressed genes (DEGs) were found in the antigen p23-silenced group, including 149 upregulated and 78 downregulated genes. GSEA analysis following antigen p23 gene knockdown identified significantly upregulated pathways associated with protein production and suppressed routes mostly correlated with ion transport, lipid metabolism, catalytic activity, protein modification, and G-protein activity. Partially silencing metalloprotease in tick midguts led to the identification of 182 DEGs, comprising 104 upregulated and 78 downregulated genes. Ablation of endogenous metalloprotease led to the upregulation of several biological and functional pathways associated with RNA splicing and significantly suppressed routes connected with detoxification, protein modification, catalytic activity and molecule binding. These findings provide valuable insights into tick physiology, providing a foundation for further research on tick-host interactions and AGS pathogenesis.
2025-11-04 | GSE291137 | GEO
Project description:Four genome assemblies of tick : Ixodes ricinus, Ixodes persulcatus, Ixodes pacificus and Ixodes hexagonus
| PRJEB67794 | ENA
Project description:The long blood feeding duration of hard ticks relies on the continuous modulation of saliva composition to manipulate host haemostatic and immune systems. While the exogenous muscarinic acetylcholine receptor (mAChR) agonist, pilocarpine, is a potent inducer of tick salivation, the underlying control mechanisms of endogenous stimulation remain unclear. Here, we characterised two pharmacologically distinct types of mAChRs (A and B) in the genome of the Ixodes ricinus tick. A combination of molecular dynamics and directed mutagenesis revealed the atypical muscarinic profile of the type-B receptor. Immunolabelling followed by in vivo pharmacology and proteomic analyses suggested that the composition of secreted saliva is a result of a complex interplay between different central neurons and specific regions of the salivary gland (SG), mediated by distinct axonal mAChR types. This system likely functions upstream of a neuropeptide signalling cascade, ultimately controlling different salivation subunits in SG. Our study describes a unique model of regulation of tick SG activity in a medically relevant tick species. This knowledge holds broader implications for understanding tick-host interactions.
2025-10-06 | PXD057920 | Pride
Project description:Ixodes ricinus Genome sequencing and assembly
| PRJNA1028502 | ENA