DrCraLaev2
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB67925 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Crataegus laevigata (midland hawthorn) genome assembly, drCraLaev2, alternate haplotype
| PRJEB67924 | ENA
Project description:Crataegus laevigata (midland hawthorn), genomic and transcriptomic data
| PRJEB65685 | ENA
Project description:Hawthorn (Crataegus spp.) is a valuable genus of medicinal and edible plants in the Rosaceae family that are rich in bioactive compounds. We applied complementary protoplast‑based single‑cell RNA sequencing (scRNA‑seq) and nucleus‑based snRNA‑seq to construct the first single‑cell atlas of hawthorn leaves. We optimized isolation protocols for two Crataegus species (C. pinnatifida var. major and C. scabrifolia), generated four single‑cell transcriptomic libraries across platforms, and profiled 32,292 high‑quality cells to explore the developmental landscape of hawthorn leaves. The cells were clustered into sixteen groups that we annotated into nine major leaf cell types (mesophyll, pavement, guard, xylem, metaxylem, phloem parenchyma, companion, sieve element, and meristematic cells), revealing extensive cellular heterogeneity and candidate marker genes. Pseudotime reconstruction revealed branched developmental trajectories, and cell‑type‑resolved profiling revealed the cell‑specific expression of flavonoid biosynthetic genes. This single‑cell atlas lays a foundation for mechanistic investigations of tissue‑specific metabolite biosynthesis and offers a cellular framework to guide future functional studies and trait improvement breeding in hawthorn.
2026-05-01 | GSE315448 | GEO
Project description:Crataegus monogyna (hawthorn), genomic and transcriptomic data
| PRJEB103900 | ENA
Project description:Here, we present the results of an integrative study that leverages naturally segregating variation and a recent, adaptive divergence event affecting seasonal timing to identify developmental mechanisms underlying diapause progression in a tephritid fly, Rhagoletis pomonella. Also called the apple maggot fly, R. pomonella is native to North America, where it infests fruits of native Crataegus (hawthorn) species throughout its range. Derived populations of the fly infest apples (Malus domesticus), and thus have evolved in the last ~250 years since apples were introduced. Many molecular studies in conjunction with mark-recapture experiments document that the populations, or host races, remain genetically distinct despite ongoing gene flow, making R. pomonella a textbook example of incipient speciation with gene flow and host associated divergence. Strong natural selection on two primary traits, host finding behavior and seasonal timing, maintain genetic divergence. Both populations (hereafter apple and haw flies) have one generation per year, with a functionally obligate pupal diapause, overwintering in the soil. Adults must emerge coincident with host fruit availability, typically a period of only a few weeks, in order to successfully oviposit into fruits. Apple flies have evolved an earlier (~3 week) emergence timing to synchronize with apples, which fruit about 3 weeks earlier than hawthorn at a typical, sympatric site in the Midwest (e.g., Michigan or Illinois, USA). We combined RNA sequencing (RNAseq), phenotyping of emergence timing and brain morphology, and whole genome pooled resequencing (Poolseq) to infer mechanisms underlying segregating variation for diapause phenology in each Rhagoletic pomonella host race, and the evolved difference in phenology between host races.
2020-08-19 | GSE140473 | GEO