Project description:The three-spined stickleback (Gasterosteus aculeatus) is an important model organism for understanding the genetic basis of adaptation in response to ecological change. We used single nucleus RNA-sequencing (snRNA-seq) to define brain cell type composition and gene expression in adult and juvenile three-spined stickleback. These data will faciliate improved understanding of how the nervous system responds/adapts to environmental factors.
Project description:Adult female three-spined stickleback fish (Gasterosteus aculeatus) were exposed to 10 individual chemicals, 26 mixtures of these chemicals, or control conditions in a flow-through system for 4 days. Transcriptomics was performed on liver samples by microarray. The main aims were to determine molecular signatures induced by these chemicals in the three-spined stickleback, discover whether these persisted in chemical mixtures and identify non-additive molecular responses in chemical mixtures exposures.
