Project description:Mycobacterium avium is the most common nontuberculous mycobacterium (NTM) species causing infectious disease. Here, we characterized a M. avium infection model in zebrafish larvae, and compared it to M. marinum infection, a model of tuberculosis. Using RNAseq analysis, we found a distinct transcriptome response in cytokine-cytokine receptor interaction for M. avium and M. marinum infection. In addition, we found substantial differences in gene expression in metabolic pathways, phagosome formation, matrix remodeling, and apoptosis in response to these mycobacterial infections.
Project description:To understand nontuberculous mycobacterial (NTM) pathogenesis, we evaluated immune responses to Mycobacterium avium (Mav) in asymptomatic individuals with a previous history of M. avium complex lung disease (MACDZ). We analyzed global gene expression in paired Mav-infected and uninfected peripheral blood monocytes from 17 MACDZ and 17 healthy controls.
Project description:Transcriptomic profiling was performed on PMA-differentiated THP-1-derived human macrophages to investigate host transcriptional responses to infection with novel mycobacterial species (Mycobacterium camsae, Mycobacterium pumcae) in comparison with known reference strains (Mycobacterium gordonae, Mycobacterium abscessus, Mycobacterium marinum). This dataset provides insights into host-pathogen interactions and identifies key differentially expressed genes and pathways involved in macrophage responses to mycobacterial infection.
2026-05-15 | GSE328953 | GEO
Project description:The lung microbiota in nontuberculous mycobacterial pulmonary disease
Project description:We performed Spatial transcriptomics (Visium) using the 10X Genomics platform to investigate the role of the autophagy-related protein ATG7 in the context of nontuberculous mycobacterial (NTM) infection. ATG7 in innate immune cells plays a critical role in controlling NTM infection and protecting lung tissue from pathological inflammation. This study represents single-cell analysis of Mice deletion of Atg7 in innate immune cells and reveals the importance of ATG7 in mediating antimicrobial responses during infection and its essential role in host defense mechanisms against NTM.
