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RNA-seq of Streptomyces coelicolor hupA and hupS deletion strain

ABSTRACT: RNA-seq of Streptomyces coelicolor hupA and hupS deletion strain

PROVIDER: PRJEB72891 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12687800_1.fastq.gz	Fastqsanger.gz
	ERR12687800_2.fastq.gz	Fastqsanger.gz
	ERR12687801_1.fastq.gz	Fastqsanger.gz
	ERR12687801_2.fastq.gz	Fastqsanger.gz
	ERR12687802_1.fastq.gz	Fastqsanger.gz

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RNA-seq of Streptomyces coelicolor hupA and hupS deletion strain

Project description:HupA and HupS are nucleoid associated proteins, homologic to E. coli HU protein. Their binding to DNA changes chromosome structure, protects DNA from damage and influences gene expression. The goal of RNA-seq experiment was to determine HupA and HupS regulons during growth in liquid medium. Dataset contains four strains (hupA, hupS, hupAhupS deletion mutants and wild type), two time points (exponential and stationary growth) and two growth conditions (standard media and osmotic stress).

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RNA-seq of Streptomyces coelicolor and Streptomyces davawensis including a dodecin gene deletion variant in response to plumbagin treatment

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Project description:Global regulation by the Streptomyces coelicolor atypical MerR-like transcription factor BldC. BldC is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldC regulon by means of chromatin immunoprecipitation (ChIP) microarray analysis. The BldC regulon encompasses at least 201 transcriptional units, which include many genes that play key roles in Streptomyces development (e.g., bldC itself, bldB, bldM, whiB, whiD, whiI, sigF, smeA-sffA, hupS), antibiotic production (e.g., afsK) and stress response (e.g., clpB, nsrR, sigE, sigF). All BldC-binding sites identified by ChIP-chip are present in the promoters of the target genes. In vitro DNA-binding experiments show that BldC is capable of binding DNA specifically in the absence of other proteins and suggest that BldC is a minor-groove DNA-binding protein. The regulon of BldC partially overlaps with that of the pleiotropic regulator BldD. BldC and BldD bind to distinct sites in the promoter region of smeA, where they simultaneously repress its transcription.

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