Project description:Analysis the sequence differences between the pair of near-isogenic lines with contrasting days to flowering to identify candidate gene variants associated with flowering time in chickpea
Project description:Analysis the sequence differences between the parental lines and one pair of near-isogenic lines with contrasting for leaf types to identify the gene responsible for the control of the compound leaf in chickpea
Project description:Evaluation of transcripts from soybean seed tissue during seed fill for a pair of near-isogenic lines contrasting in seed protein and oil and carrying an introgression at the linkage group I protein QTL region. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Yung-Tsi Bolon. The equivalent experiment is GM11 at PLEXdb.]
Project description:Evaluation of transcripts from soybean seed tissue during seed fill for a pair of near-isogenic lines contrasting in seed protein and oil and carrying an introgression at the linkage group I protein QTL region. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Yung-Tsi Bolon. The equivalent experiment is GM11 at PLEXdb.] genotype: LoProt - time: 25-50(3-replications); genotype: LoProt - time: >50-100(3-replications); genotype: LoProt - time: >100-200(3-replications); genotype: LoProt - time: >200-300(3-replications); genotype: HiProt - time: 25-50(3-replications); genotype: HiProt - time: >50-100(3-replications); genotype: HiProt - time: >100-200(3-replications); genotype: HiProt - time: >200-300(3-replications)
Project description:The primary objective of this study is to investigate the molecular differences between chickpea varieties with early and late flowering, focusing on gene expression in response to heat stress. Through the analysis of differentially expressed genes under high-temperature conditions, we aim to gain a deeper understanding of the molecular mechanisms underlying early flowering in chickpea. This approach will not only help bridge the current gaps in our scientific knowledge but may also provide valuable insights for the development of targeted genetic improvement strategies to enhance the thermal resilience of chickpea crops.
Project description:Gene expression of a near-isogenic pair of barley lines differing in absence or presence of the mlo5 resistance gene in response to attack by barley powdery mildew (Bgh). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Achim Fischer. The equivalent experiment is BB7 at PLEXdb.]
Project description:Aflatoxin contamination caused by Aspergillus flavus in peanut is a serious constraint for food safety and human health. However, molecular mechanism/s underlying the defense response is poorly understood. A comparative proteomic analysis was carried out between two contrasting peanut genotypes, JL24 (WT-susceptible) and a near-isogenic transgenic event (OE-Def) in the same background expressing defensin gene (resistant) with different time points, To understand the proteome changes in OE-Def and WT control lines, a label-free quantitative proteomics analysis was performed at 0, 24, 40, 56 and 72 h after A. flavus inoculation using UPLC-ESI-MS/MS. Several resistance proteins in the secondary metabolic pathways related to phenylpropanoids, flavonoids, and fatty acid biosynthesis were strongly induced in the resistant genotype.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from near isogenic spring wheat lines carrying different combinaison of the FHB resistance QTLs 2DL, 3BS and 5A, after inoculation with water (H2O) or Fg; the point inoculation method was used.
Project description:Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from near isogenic spring wheat lines carrying or not the FHB resistance QTL 2DL, after inoculation with water (H2O) or Fg; two inoculation methods were also compared, point and spray inoculation.