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RNA-seq of butyrate micelle or control treated murine Ly6Chi CD11b+ myeloid cells

ABSTRACT: RNA-seq of butyrate micelle or control treated murine Ly6Chi CD11b+ myeloid cells

PROVIDER: PRJEB74200 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12778884_1.fastq.gz	Fastqsanger.gz
	ERR12778884_2.fastq.gz	Fastqsanger.gz
	ERR12778885_1.fastq.gz	Fastqsanger.gz
	ERR12778885_2.fastq.gz	Fastqsanger.gz
	ERR12778886_1.fastq.gz	Fastqsanger.gz

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RNA-seq of butyrate micelle or control treated murine Ly6Chi CD11b+ myeloid cells

Project description:We aimed to study the impact of bacteria-derived metabolites in solid organ transplantation. The short-chain fatty acid butyrate was administered using a new drug-delivery method developed by Wang et al. (https://doi.org/10.1038/s41551-022-00972-5). This system is based on a micelle platform that carries and protects butyrate (ButM) from degradation in the upper gastrointestinal tract, to lower distal portions of the gut. Mice (adult B6 females) were treated for 21 days with ButM (100mg/mL) or 1x PBS. Splenic Ly6Chi CD11b+ monocytes were sorted for bulk RNAseq using the Takara SMART-Seq mRNA LP and Unique Dual Index kits. Libraries were sequenced using the Illumina NovaSeq 6000 platform.

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