Project description:The study aim was to identify novel serum markers of prostate cancer diagnosis compared to benign prostate hyperplasia and healthy controls
Project description:Single nuclear ATAC sequencing of tissue from BPH patients (n=4) that underwent elective HOLEP surgery. This data was utilised in conjunction with the single nuclear RNA data of the same tissue to identify open chromatin enrichment peaks that are associated with gene expression, and ultimately to establish an inference with GWAS variants.
Project description:Six de-identified benign prostatic hyperplasia prostate specimens were profiled by 10x Genomics single-cell RNA-seq. Samples were stratified into Large BPH and Small BPH groups by prostate weight. Raw FASTQ files and sequencing-provider CellRanger filtered feature-barcode matrices are provided; downstream quality control, integration, annotation, and endothelial-focused analyses were performed by the authors.
Project description:Single nuclear RNA sequencing of tissue from BPH patients (n=4) that underwent elective HOLEP surgery. This data was utilised in conjunction with the single nuclear ATAC data of the same tissue to identify open chromatin enrichment peaks that are associated with gene expression, and ultimately to establish an inference with GWAS variants.
Project description:Although an increased level of the prostate-specific antigen can be an indication for prostate cancer, other reasons often lead to a high rate of false positive results. Therefore, an additional serological screening of autoantibodies in patients’ sera could improve the detection of prostate cancer. We performed protein macroarray screening with sera from 49 prostate cancer patients, 70 patients with benign prostatic hyperplasia and 28 healthy controls and compared the autoimmune response in those groups. We were able to distinguish prostate cancer patients from normal controls with an accuracy of 83.2%, patients with benign prostatic hyperplasia from normal controls with an accuracy of 86.0% and prostate cancer patients from patients with benign prostatic hyperplasia with an accuracy of 70.3%. Combining seroreactivity pattern with a PSA level of higher than 4.0 ng/ml this classification could be improved to an accuracy of 84.1%. For selected proteins we were able to confirm the differential expression by using Lluminex on 84 samples. We provide a minimally invasive serological method to reduce false positive results in detection of prostate cancer and according to PSA screening to distinguish men with prostate cancer from men with benign prostatic hyperplasia.
Project description:Despite its low incidence, the aggressive nature and high metastatic potential of canine prostate cancer (PC) make it a serious problem in veterinary medicine. However, knowledge of the molecular mechanisms and regulatory networks underlying the development and progression of canine PC is still limited. We used RNAseq to identify diffentially expressed genes between PC (n=2) and benign prostate hyperplasia (BPH, n=3) samples. Based on these results, we chose 26 genes for validation on a larger patient cohorts. We used Nanosting nCounter technology for the comparative analysis of gene expression profiles in PC (n=14) and BPH (n=7) tissue samples. Finally the results for voltage-dependent calcium channel α2δ1 subunit were validated using Western blotting and immunohistochemistry.
Project description:Despite its low incidence, the aggressive nature and high metastatic potential of canine prostate cancer (PC) make it a serious problem in veterinary medicine. However, knowledge of the molecular mechanisms and regulatory networks underlying the development and progression of canine PC is still limited. We used RNAseq to identify diffentially expressed genes between PC (n=2) and benign prostate hyperplasia (BPH, n=3) samples. Based on these results, we chose 26 genes for validation on a larger patient cohorts. We used Nanosting nCounter technology for the comparative analysis of gene expression profiles in PC (n=14) and BPH (n=7) tissue samples. Finally the results for voltage-dependent calcium channel α2δ1 subunit were validated using Western blotting and immunohistochemistry.