Ontology highlight
ABSTRACT:
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR661096.fastq.gz | Fastqsanger.gz | |||
| ERR661097.fastq.gz | Fastqsanger.gz | |||
| ERR661098.fastq.gz | Fastqsanger.gz | |||
| ERR661099.fastq.gz | Fastqsanger.gz | |||
| ERR661100.fastq.gz | Fastqsanger.gz |
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Project description:Phosphorus (P) limitation will play a key role in the productivity of agriculture in the coming decades. Struvite is an ammonium magnesium phosphate mineral that can be recovered from wastewater-treatment plants and can be considered as an alternative source of P. However, the impact of struvite on the plant yield and, particularly, on the soil microbial community is barely known. Here, we tested the impacts of struvite, sewage sludge, and their combination on the barley yield, soil macro and micronutrients, and biochemical and microbiological soil properties. Amendment with struvite alone and its combination with sludge increased the availability of P in soil, the plant uptake of P and Mg, and the barley yield. The analysis of phospholipid fatty acids (PLFAs) and metaproteomics approaches revealed significant effects of struvite on the biomass of Gram-positive bacteria and, particularly, on actinobacterial populations in soil.
2019-03-13 | PXD009595 | Pride
Project description:.RAW files and Compound Discoverer peak lists used for a manuscript regarding changes the chemical fingerprint of sewage sludge during the COVID-19 pandemic
2021-01-08 | MSV000086676 | MassIVE
Project description:The effects of the antimicrobial triclosan (TCS) and its transformation product methyl-triclosan (MTCS) on the earthworm Eisenia fetida were investigated using GC-MS metabolomics. TCS is ubiquitous in sewage sludge, but a large proportion is transformed into MTCS during wastewater treatment and in soil when sewage sludge is applied to land. Our objective was to determine if earthworms exposed to ng/g to μg/g concentrations of TCS or MTCS exhibit toxic effects, and to identify the toxic mode of action of each compound. Ten individual earthworm replicates in 10 g worm bedding were exposed to 0, 0.25, 1, 4, 16, or 64 μg/g of either TCS or MTCS (120 experimental units) for 14 days. No mortalities were observed. All MTCS exposed worms had an instantaneous growth rate (IGR) over two times higher than the control during the study, but there was no effect of increasing concentration. Succinic acid was elevated relative to the control at concentrations ≥ 0.25 μg/g and glucose was elevated at 1 μg/g. There was separation from the control at all concentrations except 4 μg/g using Principal Components Analysis. Glucose, palmitic acid, and IGR contributed most strongly to the separation. Discriminant analysis with succinic acid, glucose, and IGR as variables showed a clear separation at all concentrations from the control along Canonical 1. Disruption of energy metabolism was hypothesized as a possible mode of action for MTCS.
2018-09-17 | MTBLS532 | MetaboLights
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.
2014-12-03 | GSE63781 | GEO
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers. 18 samples; Triplicate PHB-enriched bacterial communities recovered from activated sludge were exposed to nanoparticle (TiO2 or Ag) or AgNO3 (as a silver control) or were not exposed to an nanoparticles (control) to determine if the naoparticles affected PHB production.
2014-12-03 | E-GEOD-63781 | biostudies-arrayexpress
Project description:The spread of antibiotic resistance genes (ARG) into agricultural soils, products, and foods severely limits the use of organic fertilizers in agriculture. In this study, experimental land plots were fertilized, sown, and harvested for two consecutive agricultural cycles using either mineral or three types of organic fertilizers: sewage sludge, pig slurry, or composted organic fraction of municipal solid waste. The analysis of the relative abundances of more than 200,000 ASV (Amplicon Sequence Variants) allowed the identification of a small, but significant (<10%) overlap between soil and fertilizer microbiomes, particularly in soils sampled the same day of the harvest (post-harvest soils). Loads of clinically relevant ARG were significantly higher (up to 100 fold) in fertilized soils relative to the initial soil. The highest increases corresponded to post-harvest soils treated with organic fertilizers, and they correlated with the extend of the contribution of fertilizers to the soil microbiome. Edible products (lettuce and radish) showed low, but measurable loads of ARG (sul1 for lettuces and radish, tetM for lettuces). These loads were minimal in mineral fertilized soils, and strongly dependent on the type of fertilizer. We concluded that at least part of the observed increase on ARG loads in soils and foodstuffs were actual contributions from the fertilizer microbiomes. Thus, we propose that adequate waste management and good pharmacological and veterinarian practices may significantly reduce the potential health risk posed by the presence of ARG in agricultural soils and plant products.
2021-07-09 | GSE179685 | GEO
Project description:.RAW files and Compound Discoverer peak lists used for a manuscript regarding changes the chemical fingerprint of sewage sludge during the COVID-19 pandemic
2021-01-08 | MSV000086676 | GNPS
Project description:BACKGROUND: Sewage sludge (SL) and hydrochar (HC) agricultural applications are nowadays a common practice to soil conditioning and crop fertilization, however safety concerns for human and environmental health due to the presence of toxic compounds, have been recently increased. OBJECTIVES: Our aim is to assess the safety for humans and the environment of SL and derived HC applications. METHODS: We applied proteomics and bioinformatics analyses to the cell cultures used for DR-CALUX® bioassay to identify differentially abundant proteins regulated upon exposure to SL and derived HC samples rather than only using the Bioanalytical Toxicity Equivalents (BEQs) obtained by the DR- CALUX® system itself. RESULTS: DR-CALUX® cells exposed to SL or HC showed a differential protein pattern of abundance dependent on the SL and HC extracts. The modified proteins were involved in antioxidant pathways, unfolded protein response and DNA damage that have close correlations with dioxin effects on the biological system and with cancer and neurological disorder onset. Moreover, other evidence suggests the enrichment of heavy metals in extracts according to cellular response. DISCUSSION: This work represents the next step of CALUX bioassay for screening polyhalogenated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls in HC and SL and confirm to be successful in screening proteins which abundance is dependent on the SL and HC extracts in addition to the occurrence and biological activity of legacy toxic compounds not only belonging to organohalogens but still detectable for their biological activity in both matrices.
2023-06-26 | PXD040115 | Pride