Project description:A) Whole lung tissue from 24 months (n=7) and 3 months old (n=8) mice was dissociated and single-cell mRNAseq libraries generated with Drop-Seq. B) Bulk RNA-seq data was generated from whole mouse lung tissue of old (n=3) and young (n=3) samples. C) Bulk RNA-seq data was generated from flow-sorted macrophages from old (n=7) and young (n=5) mice and flow-sorted epithelial cells from old (n=4) and young (n=4) mice.
Project description:the intervention period was 4 weeks, and treadmill exercises were conducted for all exercise groups. Two sessions per day were administered. A minimum of a one-hour break was allocated between sessions. the 9-week-old C57BL/6 young male mice and 84-week-old mice were purchased from Central Lab. The groups were divided into young control (YC), young exercise (YE), old control (OC), and old exercise (OE) groups. Each group comprised three mice and all mice were subjected to RNA sequencing analysis.
Project description:The present study was to investigate the differentially expressed genes in 24-hour-old (containing proliferative cardiomyocytes), 7-day-old (containing the burst of proliferative cardiomyocytes), and 10-week-old (containing growth-arrested cardiomyocytes) C57BL/6 mouse hearts using global gene expression profiles.
Project description:This project describes the quatitative changes in the rabbit liver in response of increasing age. Quantitative proteomics workflow was used to track metabolic changes that occur in the rabbit liver with elderly. Three age groups (young, middle, and old age rabbits were quantitatively compared and enrich classes of proteins involved in mitochondrial dysfunction, protein, carbohydrate, and fat metabolism were detected.
Project description:Male Fischer 344 rats aged 4 months (young, n=10), 14 months (mid-aged, n=10), and 24 months (aged, n=10) were trained sequentially on two tasks: Morris Spatial Water Maze (SWM) and Object Memory Task (OMT). The training/testing sequence lasted 7 d, and hippocampal tissue was collected 24 hr later. Training and testing occured on each day except for days 2 and 3 of the 7 d sequence. (01/10/05: Series was updated to correct mislabeling of all sample signal values within the Young Treatment Group) Keywords = bioinformatics Keywords = gene expression Keywords = memory Keywords = synaptic plasticity Keywords = myelin Keywords = inflamation Keywords = aging Keywords: repeat sample
Project description:Loss of Phf6 prevents the functional decline and immunophenotypic changes associated with age-related, long-term repopulating hematopoietic stem cell (LT-HSC) exhaustion. To identify the underlying molecular mechanisms that account for these differences, we performed RNA-seq profiling of LT-HSCs isolated from the bone marrow of Phf6 wild-type and knock-out, young (16-week-old) and aged (24-month-old) C57BL/6 mice. Our analysis revealed that LT-HSCs isolated from 24-month-old, Phf6 knockout mice retained the molecular signatures associated with young LT-HSCs whereas LT-HSCs isolated from aged, Phf6 wild-type mice acquired signatures consistent with HSC exhaustion. Mechanistically, these data revealed important roles for key metabolic pathways including glutathione metabolism and sterol biosynthesis, as well as cell-cell interaction and signaling pathways such as the interferon and TGF-beta responses.
Project description:Studies on aging have largely included one or two OMICS layers, which may not necessarily reflect the signatures of other layers. Moreover, most aging studies have often compared very young (4-5 wks) mice with old (24 months) mice which does not reflect the aging transition after the attainment of adulthood. Therefore, we aimed to study and compared muti-OMICS aging signatures across key metabolic tissues of mature adults (6 months) and old (24 months) C57BL/6J mice (the most commonly used mouse strain). Here we compared the differentially regulated genes and enriched pathways for transcriptome, proteome and epigenome (H3K27ac, H3K4me3, H3K27me3, DNA methylation) across liver, heart, and quadriceps muscle. The major aging associated pathways cross multiple layers and tissues are decreased RNA metabolism, transcription, and translation at transcript and protein levels however increased potential of transcription at DNA methylation and H3K27ac levels.
Project description:We report comprehensive miRNA expression profiles by miRNA-seq analysis in tibialis anterior muscle and serum of a disuse-induced atrophy model, compared with young (6 months) and old (24 months) mice.
