Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing analysis of WT and Ythdf2-KO FGSCs

ABSTRACT: RNA sequencing analysis of WT and Ythdf2-KO FGSCs

PROVIDER: PRJEB77836 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA sequencing analysis of WT and Ythdf2-KO FGSCs

Project description:YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. Female germline stem cells (FGSCs) possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted RNA-seq assay in WT and Ythdf2-KO FGSCs and aimed to identified YTHDF2-responsive genes.

2025-07-26 | E-MTAB-14257 | biostudies-arrayexpress

MeRIP sequencing analysis of female germline stem cells (FGSCs)

Project description:YTHDF2 displays extensive-expression patterns during oocyte maturation and its deficiency causes female infertility in mice. However, its specific mechanism of regulation remains elusive due to the absence of suitable in vitro models. FGSCs possess the capacity for self-renewal and differentiation into oocytes to support reproduction. The successful establishment of a line of FGSCs provides a platform for scientific research on female fertility and oogenesis. To understand how YTHDF2 exerts its regulatory effects on FGSCs, we conducted MeRIP-seq assay in FGSCs and aimed to identified YTHDF2 target transcripts.

2025-07-26 | E-MTAB-14256 | biostudies-arrayexpress

MeRIP, RIP and RNA Sequencing of the H1299 stable cell lines which the endogenous YTHDF2 was silenced by a short hairpin RNA targeting YTHDF2 3’UTR (shYTHDF2) and then re-expressed Flag-tagged YTHDF2-WT orYTHDF2-K571R

Project description:SUMOylation affects many aspects of target proteins such as activity, stability, localization and protein-protein interactions. We have found that SUMOylation of YTHDF2 increased its binding activity with m6A-RNAs by using different experimental approaches. To confiremd this conclusion,the analysis of RIP-seq, MeRIP-seq and RNA-seq in H1299-shYTHDF2 cells re-expressing YTHDF2-WT and YTHDF2-K571R was performed. MeRIP+RIP targets showed lower binding affinities in the mutant YTHDF2-K571R when compared with YTHDF2-WT. Compared to the control group, the binding capacities of YTHDF2 to RIP targets in treated group with either 2-D08 or GA were decreased, especially to MeRIP+RIP targets. Moreover, SUMOylated YTHDF2 promoted m6A-RNAs degradation. Combined analysis of RNA-seq, RIP-seq and MeRIP-seq showed that the mRNA levels were up-regulated in shYTHDF2 stable cells re-expressing YTHDF2-K571R compared with those in re-expressing YTHDF2-WT.

2021-01-26 | GSE164917 | GEO

YTHDF2 controls muscle size through regulation of targeted proteostasis

Project description:Proteomics data from Muscle overload surgery in ctrl and Ythdf2-KO mice

2023-10-24 | MSV000093181 | MassIVE

WT and ATRX KO

Project description:WT and ATRX KO

| PRJNA254835 | ENA

WT/SCRN1 KO proteomic analysis

Project description:liquid chromatographytandem-mass spectrometry (LC-MS) and proteomic analysis were conducted to analyze the differential expression proteins (DEPs) in WT and SCRN1 KO cells

2023-12-10 | PXD047686 |

Microarray analysis of YTHDF2-deficient leukemic stem cells

Project description:YTHDF2 is overexpressed in a broad spectrum of human acute myeloid leukemias (AML). To study the role of YTHDF2 in leukemia, total RNA from Ythdf2CKO (n=4) and Ythdf2CTL (n=4) leukemic stem cells were used for Affymetrix global gene expression analysis.

2019-04-05 | E-MTAB-7782 | biostudies-arrayexpress

mRNAseq and proteomic data set of one week old WT (Chop wt/wt CkmmCre wt/wt Dars2 fl/fl), Chop KO (Chop ko/ko CkmmCre wt/wt Dars2 fl/fl), Dars2 KO (Chop wt/wt CkmmCre tg/wt Dars2 fl/fl) and DKO (Chop ko/ko CkmmCre tg/wt Dars2 fl/fl) mice

Project description:mRNAseq and proteomic data set of one week old WT (Chop wt/wt CkmmCre wt/wt Dars2 fl/fl), Chop KO (Chop ko/ko CkmmCre wt/wt Dars2 fl/fl), Dars2 KO (Chop wt/wt CkmmCre tg/wt Dars2 fl/fl) and DKO (Chop ko/ko CkmmCre tg/wt Dars2 fl/fl) mice

| PRJNA627135 | ENA

Genome-wide maps of chromatin state in YTHDF2 kockdown(KD) and wildtype(WT) cells in Multiple Myeloma

Project description:We found that the experssion of STAT5A was upregulated when the YTHDF2 was knocked down. To identify the function of STAT5A protein as a Transcription factor in YTHDF2 kockdown(KD) and wildtype(WT) cells in Multiple Myeloma. CHIP-seq was conducted to analyze changes in related signaling pathways and gene expression in Multiple Myeloma cells when YTHDF2 was knocked down.

2022-06-30 | GSE163138 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and YTHDF2 KD human umbilical cord blood CD34+ cell Transcriptomes

Project description:RNA-seq analysis were performed with total RNA extracted from wt and YTHDF2 KD human UCB CD34+ cells.

2018-08-17 | GSE107956 | GEO