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IlLasQuer

ABSTRACT: Lasiocampa quercus (oak eggar)

PROVIDER: PRJEB79192 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	ERR13605509.fastq.gz	Fastqsanger.gz
	ERR13602172_1.fastq.gz	Fastqsanger.gz
	ERR13602172_2.fastq.gz	Fastqsanger.gz

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ilLasQuer

Project description:Lasiocampa quercus (oak eggar), genomic and transcriptomic data

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [miRNA-seq]

Project description:Identification of genes involved in the regulation of taproot and lateral root growth in Quercus robur seedlings under drought stress and well-watered conditions. Genes involved in the regulation of taproot and lateral root growth in Quercus robur seedlings were identified using RNA-seq, miRNA-seq, and degradome-seq. The analysis focused on the gene expression, miRNA regulation, and mRNA degradation profiles of taproots and lateral roots under both drought stress and well-watered conditions. Key genes and their regulatory miRNAs were identified, along with the role of mRNA degradation pathways in response to stress, providing insights into the molecular mechanisms controlling root growth and development in oak seedlings under varying water availability.

2025-04-22 | GSE295320 | GEO

The impact of drought on phellem development: morpho-physiological adaptations and gene expression dynamics in cork oak stems

Project description:This work aimed to characterize the molecular adaptations occurring in cork oak (Quercus suber) stems in adaptation to drought, and identify key genetic pathways regulating phellem development. One-year-old cork oak plants were grown for additional 6 months under well-watered (WW) or water-deficit (WD) conditions and main stems were targeted for transcriptomic analysis. WD had a negative impact on secondary growth, decreasing the activity of the vascular cambium and phellogen. Following a tissue-specific approach, we analyzed the transcriptional changes imposed by WD in phellem (outer bark), inner bark, and xylem, and found a global downregulation of genes related to cell division, cell wall biogenesis, lignin and/or suberin biosynthesis. Phellem and phloem showed a concerted upregulation of photosynthesis-related genes, suggesting a determinant role of stem photosynthesis in the adaptation of young plants to long-term drought. The data gathered will be important to further harness the diverse genetic background of this species for the development of optimized management practices.

2024-04-29 | E-MTAB-13376 | biostudies-arrayexpress

RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [degradome]

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RNA-seq, miRNA-seq, and degradome-seq analysis of taproots and lateral roots of Quercus robur seedlings under well-watered and drought stress conditions [RNA-seq]

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2-DE proteomics analysis of drought treated seedlings of Quercus ilex

Project description:Applying a gel-based proteomic approach, the dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and subjected to two-dimensional electrophoresis. Coomassie colloidal stained gel images were analysed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in the three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Taxonomy Holm oak (Quercus ilex subsp. Ballota [Desf.] Samp.). Dentro de Q. ilex hay dos subespecies, ilex ilex e ilex Ballota.

2015-09-09 | PXD002484 | Pride

The Holm oak (Quercus ilex) proteome as defined by shotgun MS (nLC-MS/MS, Orbitrap) approach

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