Project description:Zaire ebolavirus: Genome sequencing by SP_NML_PHAC, National Microbiology Laboratory - Public Health Agency of Canada, Winnipeg, Canada
Project description:Monkeypox virus: Genome sequencing by SP_NML_PHAC, National Microbiology Laboratory - Public Health Agency of Canada, Winnipeg, Canada
| PRJEB87374 | ENA
Project description:Whole genome-based public health surveillance of STEC
| PRJEB32361 | ENA
Project description:Cholera surveillance activities from Ethiopian Public Health Labs
| PRJNA1111822 | ENA
Project description:Salmonella Agona molecular typing by Next Generation Sequencing for molecular surveillance
Project description:Chronic pain is a global public health problem, but the underlying molecular mechanisms are not fully understood. Here we examine genome-wide DNA methylation, first in 50 identical twins discordant for heat pain sensitivity and then in 50 further unrelated individuals. Whole blood DNA methylation was characterized at 5.2 million loci by MeDIP-sequencing and assessed longitudinally to identify differentially methylated regions associated with high or low pain-sensitivity (pain-DMRs). Nine meta-analysis pain-DMRs show robust evidence for association (false discovery rate 5%) with the strongest signal in the pain gene TRPA1 (P=1.2M-CM-^W10-13). Several pain-DMRs show longitudinal stability consistent with susceptibility effects, have similar methylation levels in brain, and altered expression in skin. Our approach identifies epigenetic changes in both novel and established candidate genes that provide molecular insights into pain and may generalize to other complex traits. MeDIP-sequencing in 100 individulas using a 2 stage design: paired-end MeDIP-seq in 50 monozygotic twins and single-end MeDIP-seq in 50 unrelated individuals.
