Project description:Genomic content of Bordetella pertussis clinical isolates circulating in areas of intensive children vaccination. 13 isolates and one reference strain of Bordetella pertussis used.
Project description:Proteomic expression data was integrated into a manually curated base Bordetella pertussis metabolic model (iBP1870). This study included preoteomic expression from cells grown in biofilm and planktonic conditions. Also included are models created using expression data de Gouw et al. Bordetella pertussis biofilm studies. Models were generated using the iMAT implemented in the Cobra Toolbox.
Project description:Murine lung gene expression responses to primary and secondary infection with Bordetella pertussis. Data were compared to other parameters such as flow cytometry and multiplex immunoassays.
Project description:Bordetella pertussis is a Gram-negative coccobacillus that causes whooping cough or pertussis, a respiratory disease that has recently experienced a resurgence. Upon entering the respiratory tract, B. pertussis colonizes the airway epithelium and attaches to ciliated cells. Here, we used primary human nasal epithelial cells (hNECs) cultured at the air-liquid interface, and investigated their transcriptomic response after infection with the B. pertussis B1917. We also analyzed role of the type III secretion system effector protein BteA in modulating this response.
Project description:Bordetella pertussis is a Gram-negative coccobacillus that causes whooping cough or pertussis, a respiratory disease that has recently experienced a resurgence. Upon entering the respiratory tract, B. pertussis colonizes the airway epithelium and attaches to ciliated cells. Here, we used primary human nasal epithelial cells (hNECs) cultured at the air-liquid interface, and investigated their transcriptomic response after infection with the B. pertussis B1917. We also analyzed role of the type III secretion system effector protein BteA in modulating this response.
