Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cervicovaginal microbiome and host immune signatures associated with pelvic inflammatory disease 16s data

ABSTRACT: Cervicovaginal microbiome signatures associated with pelvic inflammatory disease 16s data

PROVIDER: PRJEB80350 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR13719800_1.fastq.gz	Fastqsanger.gz
	ERR13719800_2.fastq.gz	Fastqsanger.gz
	ERR13719801_1.fastq.gz	Fastqsanger.gz
	ERR13719801_2.fastq.gz	Fastqsanger.gz
	ERR13719802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 268

Similar Datasets

Cervicovaginal microbiome and host immune signatures associated with pelvic inflammatory disease

Project description:Cervicovaginal microbiome signatures associated with pelvic inflammatory disease

| PRJEB80512 | ENA

The effects of host-microbe interactions on the epigenetic signatures of cervicovaginal epithelial cells [ATAC-Seq]

Project description:To identify chromatin alterations by host-microbe interactions in cervicovaginal epithelial cells we performed ATAC-sequencing. We identified regions of chromatin that were altered in cervicovaginal epithelial cells after exposure to L. crispatus supernatant.

2023-12-14 | GSE233444 | GEO

The effects of host-microbe interactions on the transcriptomic signatures of cervicovaginal epithelial cells [RNA-seq]

Project description:To elucidate the molecular pathways altered by host-microbe interactions in cervicovaginal epithelial cells we performed whole genome RNA-sequencing. We identified genes and functional pathways that were altered in cervicovaginal epithelial cells after exposure to G. vaginalis or L. crispatus or their supernatant.

2023-12-14 | GSE234836 | GEO

Lactobacillus spp isolated from cervicomicrobiome pelvic inflammatory disease study

Project description:Lactobacillus spp from pelvic inflammatory disease

| PRJEB80538 | ENA

Blood Transcriptional Endotypes in Women with Pelvic Inflammatory Disease

Project description:Blood Transcriptional Endotypes in Women with Pelvic Inflammatory Disease

| PRJNA432897 | ENA

Gene expression profile in pelvic organ prolapse

Project description:Introduction and Hypothesis: Identify processes contributing to pelvic organ prolapse (POP) by transcriptional profiling of pelvic connective tissue in conjunction with light microscopy. Methods: We performed a frequency matched case-control study of women undergoing hysterectomy. Total RNA, extracted from uterosacral and round ligament samples used to generate labeled cRNA, was hybridized to microarrays and analyzed for the expression of 32,878 genes. Significance Analysis of Microarrays, (Stanford University, CA), identified differentially expressed genes used for ontoanalysis, and quantitative PCR (qPCR) confirmed results. Light microscopy confirmed tissue type and assessed inflammatory infiltration. Results: The analysis of thirty-four arrays revealed 249 differentially expressed genes with fold changes larger than 1.5 fold and false discovery rates M-bM-^IM-$5.2%. Immunity and Defense was the most significant biological process differentially expressed in POP. Selected qPCR confirmed 4 genes. Light microscopy showed no inflammatory infiltrates. Conclusions: Genes enriched for Immunity and Defense contribute to POP independent of inflammatory infiltrates. Keywords: whole tissue (endopelvic fascia) type comparison This was a group matched case control study of 8 women with pelvic organ prolapse versus 9 non-prolapse controls, both undergoing hysterectomy for benign conditions. Two separate pelvic support tissues were collected from each patient. The uterosacral ligament and round ligament tissue was removed at the time of hysterectomy, RNA was extracted and ABI whole genome chips used to identify differences in expression profiles of individual samples. Various ethnic groups, age groups and menopausal status were included.

2008-09-19 | E-GEOD-12852 | biostudies-arrayexpress

Cervicovaginal dysbiosis is associated with significant changes in the cervicovaginal proteome related to immune activation and increased cell death

Project description:Cervicovaginal microbiome dysbiosis is associated with increased prevalence and incidence of sexually transmitted infections including HIV. We compared the cervicovaginal proteome as characterised by mass-spectrometry of four groups of African female sex workers (total N=50) grouped by microbiome composition as characterised by 16S rDNA microarray. Group 1 had a Lactobacillus crispatus-dominated microbiome, group 2 a L. iners-dominated microbiome, and groups 3 and 4 had a microbiome containing multiple genera of anaerobic bacteria, with group 3 representing transition to or from dysbiosis and group 4 full dysbiosis. 82 human proteins were differentially abundant among the groups, either showing an increasing or decreasing trend from microbiome groups 1 to 4. Proteins that increased included proteasome subunits and other proteins involved in catabolic metabolism, actin organising proteins and proteins involved in the immune response. Proteins that decreased included antiproteases, keratins, and cornified envelope proteins. We also compared the abundance of pre-defined proteins of interest among microbiome groups: markers of cell type, inflammation, and cell death, and mucins. The dysbiotic groups had increased abundance of proteins unique to lymphocytes and macrophages, pro-inflammatory cytokines, cell death markers, and MUC5B. We conclude that the cervicovaginal human proteome is associated with the cervicovaginal microbiome in a dose-response manner. The changes are likely caused by a pro-inflammatory influx of immune cells and an increase of cell death in dysbiosis. Dysbiosis-associated immune activation, breaches in epithelial integrity, altered mucin balance, and altered protease-antiprotease balance may all contribute to the increased risk of HIV transmission when cervicovaginal dysbiosis is present.

2015-09-25 | PXD001435 | Pride

Blood Transcriptional Endotypes in Women with Pelvic Inflammatory Disease

Project description:By comparing blood mRNA responses of women with biopsy confimed Chlamydia trachomatis (CT) and/or Neisseria gonorrhoeae (GC) induced pelvic inflammatory disease (PID) to those from women with CT and/or GC infection limited to their cervix and asymptomatic uninfected women determined via microarray, we discovered important pathogenic mechanisms in PID and response differences that provide a pathway to biomarker discovery. Women with GC and/or CT-induced PID exhibit overexpression of myeloid cell genes and suppression of protein synthesis, mitochondrial oxidative phosphorylation, and T-cell specific genes. Coinfected women exhibited the greatest activation of cell death pathways and suppression of responses essential for adaptive immunity. Blood microarrays reveal discrete pathobiological endotypes in women with PID that are driven by pathogen invasion of the upper genital tract.

2018-02-06 | GSE110106 | GEO

Role of cervicovaginal microbiota in genital inflammation

Project description:We performed gene expression analysis using the SCRB-Seq method from cervical and peripheral blood antigen presenting cells that were collected from women with different cervicovaginal bacterial community types.

2015-05-19 | GSE68452 | GEO

The effects of host-microbe interactions on the epigenetic signatures of cervicovaginal epithelial cells [ATAC-Seq]

Project description:The effects of host-microbe interactions on the epigenetic signatures of cervicovaginal epithelial cells [ATAC-Seq]

| PRJNA976279 | ENA