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KaAstHumi

ABSTRACT: Asterocarpa humilis ((an Acsidian)), genomic and transcriptomic data

PROVIDER: PRJEB80498 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR13731935_1.fastq.gz	Fastqsanger.gz
	ERR13731935_2.fastq.gz	Fastqsanger.gz
	ERR13725992.fastq.gz	Fastqsanger.gz
	ERR13962549_1.fastq.gz	Fastqsanger.gz
	ERR13962549_2.fastq.gz	Fastqsanger.gz

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kaAstHumi

Project description:Asterocarpa humilis ((an Acsidian))

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Activation of seed-specific genes in leaves and roots of the desiccation-tolerant plant, Xerophyta humilis

Project description:Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in desiccated (5% RWC) and hydrated (100% RWC) Xerophyta humilis leaves and roots, and in mature seeds. 3105 cDNA clones (corresponding to 1709 unique cDNAs) were randomly selected from X. humilis cDNA libraries (Leaf Dehydration (LD), Leaf Rehydration (LR), Root Dehydration (RD) and Root Rehydration (RR)), amplified by PCR, and printed on each glass slide multiple times (ranging from 4 to 12X), such that each printed block contained a random unbiased mixture of cDNAs from the 4 different cDNA libraries. Total RNA extracted from each X. humilis leaf, root and seed sample was labelled with Cy3 and hybridized to the printed cDNA arrays. Data was recorded for three biological replicates for each of the samples being investigated.

2012-03-29 | E-GEOD-34951 | biostudies-arrayexpress

The transcriptome response of leaves of the resurrection plant Xerophyta humilis to desiccation

Project description:Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in Xerophyta humilis leaves at 6 relative water contents (RWC) (100%, 80%, 60%, 40%, 20% and 5%RWC respectively). 3105 cDNA clones (corresponding to 1709 unique cDNAs) were randomly selected from X. humilis cDNA libraries (Leaf Dehydration (LD), Leaf Rehydration (LR), Root Dehydration (RD) and Root Rehydration (RR)), amplified by PCR, and printed on each glass slide multiple times (ranging from 4 to 12X), such that each printed block contained a random unbiased mixture of cDNAs from the 4 different cDNA libraries. Total RNA extracted from each X. humilis leaf sample was linearly amplified, labelled with Cy3, and simultaneously hybridized with Cy5 labelled common reference RNA, to the printed cDNA arrays. The reference RNA comprised equal amounts of total RNA pooled from 6 different RWC experimental samples. Data was recorded for five biological replicates for each of the 6 RWC stages being investigated.

2012-03-29 | E-GEOD-34952 | biostudies-arrayexpress

Activation of seed-specific genes in leaves and roots of the desiccation-tolerant plant, Xerophyta humilis

Project description:Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in desiccated (5% RWC) and hydrated (100% RWC) Xerophyta humilis leaves and roots, and in mature seeds.

2012-03-30 | GSE34951 | GEO

The transcriptome response of leaves of the resurrection plant Xerophyta humilis to desiccation

2012-03-30 | GSE34952 | GEO

kaDipList

Project description:Diplosoma listerianum ((an Acsidian))

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kaDipSpon

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Response of Endozoicomonas to coral host cues and thermal stress

Project description:Endozoicomonas are prevalent, abundant bacterial associates of marine animal hosts, including corals. Their role in holobiont health and functioning, however, remains poorly understood. To identify putative interactions within the coral holobiont, we characterized a novel Endozoicomonas isolate and assessed its transcriptomic and proteomic responses to tissue extracts of its native host, the Red Sea coral Acropora humilis, at control and elevated temperatures. We show that host cues stimulated differential expression of genes assumed to be involved in the modulation of the host immune response by Endozoicomonas, such as flagellar assembly genes, ankyrins, ephrins, and serpins. Proteome analysis revealed the upregulation of vitamin B1 and B6 biosynthetic as well as glycolytic processes by Endozoicomonas in response to host cues. We further demonstrate that the inoculation of A. humilis with its native Endozoicomonas strain resulted in enhanced holobiont health metrics, such as host tissue protein content and algal symbiont photosynthetic efficiency. Behavioral, physiological, and metabolic changes in Endozoicomonas may be key to the onset and function of mutualistic interactions within the coral holobiont, and our results suggest that the priming of Endozoicomonas to a symbiotic lifestyle may involve modulation of host immunity and the exchange of essential metabolites with other holobiont members. Consequently, Endozoicomonas presumably plays an important role in holobiont nutrient cycling and may therefore be implicated in its health, acclimatization, and ecological adaptation.

2022-08-12 | PXD027178 | Pride

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