ABSTRACT: Representative isolates of Rhizobium anhuiense sp. nov., isolated from effective nodules of Vicia faba and Pisum sativum grown in Southern China
Project description:Symbiotic nitroegn fixation in functional (Fix+) and non-functional (Fix-) nodules of Vicia faba infected with Rhizobium leguminosarum was investigated using label-free shotgun tandem MS. Proteins involved in symbiotic nitrogen fixation and maintenance of the symbiosis were identified.
Project description:Rhizobium leguminosarum bv viciae strain 3841 was inoculated onto pea (Pisum sativum) seeds and nodules were harvested at 28 d. The gene expression was compared to free-living bacteria grown on succinate ammonia AMS medium.
Project description:We studied potentially amyloidogenic proteins (e.g. protein forming polymers and complexes that are resistant to treatment with ionic detergents) in root nodules formed by two lines of garden pea (P. sativum L.): Sprint-2 (Fix+ phenotype) and Sprint-2Fix- (sym31) (Fix- phenotype) inoculated with the Rhizobium leguminosarum bv. viciae RCAM1026 root nodule bacteria. The Fix+ phenotype is characterized by effective (ability to fix nitrogen) root nodules formation. The Fix- line is a descendant of the Fix+ line and forms ineffective root nodules (unable to fix nitrogen) with undifferentiated bacteroids. We demonstrated the presence of both plant and bacterial proteins in detergent resistant fractions, including previously identified amyloid proteins RopA and RopB of R. leguminosarum and vicilin of P. sativum L.
Project description:In monocarpic plants, after the production of a certain number of fruits/seeds, the activity of the Shoot Apical Meristem (SAM) and of the meristems of the secondary axes stop in a coordinated way, leading to the cessation of flower production. This phenomenon has been called Proliferative Arrest (PA). It can be assumed as a general phenomenon in monocarpic plants, as it has been described in different species of this economically important group of plants. Studies in the model species Arabidopsis thaliana and Pisum sativum have allowed the identification of factors that affect PA timing. One of them is the production of fruits and seeds. With this RNA-seq, we have identified factors that contribute to the modulation of PA in a seed-dependent manner in Pisum sativum.
