Human Gut Archaea collection from Estonian population
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ABSTRACT:
PROVIDER: PRJEB81541 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Within the gut microbiome, Methanobrevibacter and Methanosphaera species are the prevailing methanogenic archaea. In general, these archaeal species interact widely with other members of the gut microbiome, subsequently facilitating the processes of digestion and fermentation within humans, thereby playing a significant role in the gut. Despite their significance, detailed characteristics and microbiome-host interactions remain largely unexplored. One potential mechanism for microbiome-host interaction and communication involves extracellular vesicles, which play a crucial role in both inter- and intra-kingdom interactions as well as intercellular communication. The production of extracellular vesicles has been confirmed for representatives of all three domains of life, eukaryotes, bacteria, and archaea. In this study, we report for the first time that human gut-derived archaea are capable of producing extracellular vesicles. Here, we present the ultrastructure, composition, proteome, and metabolome of these newly discovered archaeal extracellular vesicles (AEV) of M. smithii (strains ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae. Here, we describe their morphology, contents of archaeal extracellular vesicles (AEV) produced by the major methanogenic archaea of the human gut, namely Methanobrevibacter smithii (strains ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae. We also describe their interaction with human cell lines and ability to trigger immune responses. The findings show a high similarity of AEVs to their bacterial counterparts in size, morphology, and composition. Proteome and metabolome analysis demonstrate high similarities between vesicles derived from Methanobrevibacter species and are highly enriched in adhesin or adhesin-like proteins, suggesting an important role for archaeal-bacterial and archaeal-host interactions. Unless the specific role of AEVs could not be identified, their production itself suggests an intricate network of interdomain interactions shaping the dynamics of the human microbiome.
2025-06-10 | PXD053245 | Pride
Project description:Chromosome segregation is a vital process for all organisms. The mechanisms underpinning chromosomal partitioning in the archaeal domain remain elusive. Our group has identified the first chromosome segregation system in thermophilic archaea. Sulfolobus solfataricus partition system consists of SegA, an orthologue of bacterial Walker-type ParA proteins; SegB, an archaea-specific DNA binding protein and a cis-acting DNA region. ChIP-seq experiments disclosed multiple SegB binding sites scattered over the chromosome and revealed a novel DNA binding motif.
2025-06-25 | GSE169604 | GEO
Project description:<p>Gastrointestinal bacteria interact with the host and each other through various mechanisms, including the production of extracellular vesicles (EVs). However, the composition and potential roles of EVs released by gut archaea are poorly understood. Here, we study EVs produced by four strains of human gut-derived methanogenic archaea: Methanobrevibacter smithii ALI, M. smithii GRAZ-2, Candidatus Methanobrevibacter intestini and Methanosphaera stadtmanae. The size (~130 nm) and morphology of these EVs are comparable to those of bacterial EVs. Proteomic and metabolomic analyses reveal that the archaeal EVs are enriched in putative adhesins or adhesin-like proteins, free glutamic and aspartic acid, and choline glycerophosphate. The archaeal EVs are taken up by macrophages in vitro and elicit species-specific responses in immune and epithelial cell lines, including production of chemokines such as CXCL9, CXCL11 and CX3CL1. The EVs produced by Ca. M. intestini strongly induce pro-inflammatory cytokine IL-8 in epithelial cells. Future work should examine whether archaeal EVs play roles in the interactions of archaea with other gut microbes and with the host.</p>
2025-07-01 | MTBLS12422 | MetaboLights