Project description:A quantitative label-free proteome analysis of cystitis and high-grade bladder cancer tissue priorly annotated and isolated via FTIR-guided lasermicrodissection.

2019-08-13 | PXD009203 | Pride

FTIR, XPol, and LC-MS confirm endogenous collagen in Edmontosaurus fossil bone C4PR_LIV

Project description:Reports of proteins in fossilized bones have ignited an on-going debate because previously it was generally assumed that fossilization results in destruction of all organic components. We combine Attenuated Total Reflection (ATR) Fourier Transform InfraRed (FTIR) spectroscopy, cross-polarized light microscopy (XPol), and two variations of Liquid Chromatography Mass Spectrometry (LC-MS), to detect and verify endogenous collagen protein in fossilized bone. Data from an Edmontosaurus dinosaur excavated from Upper Cretaceous strata of the South Dakota Hell Creek Formation were compared with that from thermally decayed modern turkey (Meleagris) bone. The four methods show different evidence for collagen presence in the fossilized bone. ATR-FTIR spectra show a signal for carbonyl (C=O) groups, a repeat moiety in protein amino acid sequences. XPol shows the birefringence of collagen within Meleagris, as well as Edmontosaurus. LC-MS bottom-up proteomics showed m/z agreement between multiple ions from trypsin-digested samples and identified peptide sequences thus far only identified in a hadrosaur. Tandem LC-MS unambiguously identified hydroxyproline, a unique collagen-indicator amino acid, in acid-digested samples from the same.

2025-03-24 | PXD048810 | Pride

Detection SARS-CoV-2 infection in mice by ATR-FTIR

Project description:Vero-E6 cell supernatant, mice lavage and human saliva were evaulated after exposure to SARS-CoV-2 via ATR-FTIR spectoscopy. To provide further information about the physiological response, proteins contained in the mouse lavage were assessed via shotgun proteomics (data shown here). Mice were exposed to active SARS-CoV-2 or UV-inactivated SARS-CoV-2 for up to 4 days, lavage collected at day 0, day 2 and day 4 (D4). Lavage proteins were pooled for day 0 (due to low protein abundance) and compared to UV-I-SARS-CoV-2(n=4)and SARS-CoV-2(n=8)samples.

2021-12-17 | PXD030012 | Pride

FTIR-based serotyping of Streptococcus pneumoniae clinical isolates

Project description:Whole-genome sequencing data from invasive Streptococcus pneumoniae isolates used to evaluate FTIR-based serotyping against WGS and PCR-hybridization.

| PRJEB121064 | ENA

Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium

Project description:Vancomycin-resistant Enterococcus faecium (VREfm) is a leading cause of healthcare-associated infections globally and demands new approaches for treatment. Here we show that genetic and pharmacological inactivation of a highly conserved NlpC/P60 peptidoglycan hydrolase, secreted antigen A (SagA), enhanced vancomycin susceptibility of VREfm ex vivo and in vivo. Notably, genetic deletion of sagA impaired VREfm peptidoglycan remodeling, growth and increased the activity of vancomycin. We then identified first-in-class covalent NlpC/P60 peptidoglycan hydrolase inhibitors and demonstrated that pharmacological inactivation of SagA activity also impaired peptidoglycan remodeling and increased the efficacy of vancomycin in several VREfm clinical isolates. Our study reveals peptidoglycan hydrolases are druggable targets whose inactivation improves the efficacy of vancomycin against VREfm.

2026-04-13 | PXD075040 | Pride

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