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Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients

ABSTRACT: Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients

PROVIDER: PRJEB82999 | ENA |

REPOSITORIES: ENA

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Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients

Project description:This experiment focused on studying the impact of myocardial infarction on bone marrow HSPCs (LineageNeg, CD38Neg, CD34Pos). Human sternal bone marrow was extracted from the sternum of acute patients who had undergone myocardial infarction (n = 6), as well as from control patients (n = 7), during surgical procedures.

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Project description:Despite a substantial progress in diagnosis and therapy, acute myocardial infarction (MI) is a major cause of mortality in the general population. A novel insight into the pathophysiology of myocardial infarction obtained by studying gene expression should help to discover novel biomarkers of MI and to suggest novel strategies of therapy. The aim of our study was to establish gene expression patterns in leukocytes from acute myocardial infarction patients. ST-segment elevation myocardial infarction alters expression of several groups of genes. On admission, several genes and pathways that could be directly or indirectly linked with lipid/glucose metabolism, platelet function and atherosclerotic plaque stability were affected (signaling of PPAR, IL-10, IL-6). Analysis at discharge highlighted specific immune response (upregulation of immunoglobulins). Highly significant and substantial upregulation of SOCS3 and FAM20 genes expression in the first 4-6 days of myocardial infarction in all patients is the most robust observation of our work Twenty-eight patients with ST-segment elevation myocardial infarction (STEMI) were included. The blood was collected on the 1st day of myocardial infarction, after 4-6 days, and after 6 months. Control group comprised 14 patients with stable coronary artery disease (CAD), without history of myocardial infarction. Gene expression analysis was performed with Affymetrix GeneChipM-BM-. Human Gene 1.0 ST microarrays and GCS3000 TG system.

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Transcription profiling of human nucleated bone marrow cells and peripheral blood leukocytes collecte from patients after myocardial infarction

Project description:Bone marrow and peripheral blood were obtained from patients after myocardial infarction. Erythrocytes were removed by NH4CL lysis to finally obtain nucleated bone marrow cells and peripheral blood leukocytes. After RNA isolation, RNA from three patients was pooled and the RNA expression profile of both organism parts were compared.

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Mouse myocardial infarction

Project description:Mouse myocardial infarction

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Single-cell RNA sequencing of human bone marrow monocytes post-myocardial infarction at acute and chronic timepoints

Project description:This experiment focused on studying the impact of myocardial infarction on bone marrow monocytes. Human sternal bone marrow was extracted from the sternum of acute and chronic patients who had undergone myocardial infarction, as well as from control patients, during surgical procedures. The samples underwent Ficoll separation and were subsequently frozen for preservation. On the day of sorting, the samples were thawn, and a total of 3,000 - 20,000 human bone marrow monocytes (Lineage-, CD45+, HLA-DR+) were sorted in 2% BSA in PBS in two biological replicates. scRNA-seq was performed.

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myocardial infarction in Reg3beta knock-out mice

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Altered gene expression pattern in peripheral blood mononuclear cells in patients with acute myocardial infarction

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