Developmental DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB83043 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| ERR14020671_1.fastq.gz | Fastqsanger.gz | |||
| ERR14020671_2.fastq.gz | Fastqsanger.gz | |||
| ERR14020672_1.fastq.gz | Fastqsanger.gz | |||
| ERR14020672_2.fastq.gz | Fastqsanger.gz | |||
| ERR14020673_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 28 |
Similar Datasets
Project description:RNA samples to pair with methylation data from the Developmental DNA Methylation in the Parasitoid Wasp Nasonia vitripennis paper
2024-12-16 | E-MTAB-14657 | biostudies-arrayexpress
Project description:For many behaviours studied at the phenotypic level, we have little or no idea of where to start searching for “candidate” genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as deciding on clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a “DEEP-Sage” gene expression approach on the Illumina sequencing platform.
2013-07-22 | GSE43352 | GEO
Project description:The parasitoid wasp Nasonia vitripennis is a major insect model for morphology, behavior, and genetics. It has haplodiploid sex determination: unfertilized eggs develop into haploid males and fertilized eggs develop into diploid females. Additionally, polyploids (diploid males, triploid females) occur in Nasonia, representing gene expression mechanisms regulating sexual maintenance outside of standard haplodiploidy. Here, we generated a transcriptomic atlas for N. vitripennis across two tissues (head and abdomen) for typical haploid males and diploid females and polyploid diploid males and triploid females. These data are a resource for analyses on transcriptomic differences across sex, tissue type, ploidy, strain, and temporal change.
2026-07-09 | GSE337527 | GEO
Project description:The parasitoid wasp Nasonia vitripennis is a major insect model for morphology, behavior, and genetics. It has haplodiploid sex determination: unfertilized eggs develop into haploid males and fertilized eggs develop into diploid females. Additionally, polyploids (diploid males, triploid females) occur in Nasonia, representing gene expression mechanisms regulating sexual maintenance outside of standard haplodiploidy. Here, we generated a transcriptomic atlas for N. vitripennis across two tissues (head and abdomen) for typical haploid males and diploid females and polyploid diploid males and triploid females. These data are a resource for analyses on transcriptomic differences across sex, tissue type, ploidy, strain, and temporal change.
2026-07-09 | GSE337526 | GEO
Project description:The parasitoid wasp Nasonia vitripennis is a major insect model for morphology, behavior, and genetics. It has haplodiploid sex determination: unfertilized eggs develop into haploid males and fertilized eggs develop into diploid females. Additionally, polyploids (diploid males, triploid females) occur in Nasonia, representing gene expression mechanisms regulating sexual maintenance outside of standard haplodiploidy. Here, we generated a transcriptomic atlas for N. vitripennis across two tissues (head and abdomen) for typical haploid males and diploid females and polyploid diploid males and triploid females. These data are a resource for analyses on transcriptomic differences across sex, tissue type, ploidy, strain, and temporal change.
2026-07-09 | GSE337529 | GEO
Project description:The parasitoid wasp Nasonia vitripennis is a major insect model for morphology, behavior, and genetics. It has haplodiploid sex determination: unfertilized eggs develop into haploid males and fertilized eggs develop into diploid females. Additionally, polyploids (diploid males, triploid females) occur in Nasonia, representing gene expression mechanisms regulating sexual maintenance outside of standard haplodiploidy. Here, we generated a transcriptomic atlas for N. vitripennis across two tissues (head and abdomen) for typical haploid males and diploid females and polyploid diploid males and triploid females. These data are a resource for analyses on transcriptomic differences across sex, tissue type, ploidy, strain, and temporal change.
2026-07-09 | GSE337528 | GEO
Project description:Whole Genome Bisulphite Sequencing of Nasonia vitripennis in the embryo, larval, prepupal, pupal and adult stages of development.
2024-12-16 | E-MTAB-14656 | biostudies-arrayexpress
Project description:Background The generalist dipteran pupal parasitoid Nasonia vitripennis injects 79 venom peptides into the host before egg laying. This venom induces several important changes in the host, including developmental arrest, immunosuppression, and alterations to normal metabolism. It is hoped that diverse and potent bioactivities of N. vitripennis venom provide an opportunity for the design of novel acting drugs. However, currently very little is known about the individual functions of N. vitripennis venom peptides and less than half can be bioinformatically annotated. The paucity of annotation information complicates the design of studies that seek to better understand the potential mechanisms underlying the envenomation response. Although the RNA interference system of N. vitripennis provides an opportunity to functionally characterise venom encoding genes, with 79 candidates this represents a daunting task. For this reason we were interested in determining the expression levels of venom encoding genes in the venom gland, such that this information could be used to rank candidate venoms. To do this we carried out deep sequencing of the transcriptome of the venom gland and neighbouring ovary tissue and used RNA-seq to measure expression from the 79 venom encoding genes. The generation of a specific venom gland transcriptome dataset also provides further opportunities to investigate novel features of this highly specialised organ. Results High throughput sequencing and RNA-seq revealed that the highest expressed venom encoding gene in the venom gland was a serine protease called Nasvi2EG007167, which has previously been implicated in the apoptotic activity of N. vitripennis venom. As expected the RNA-seq confirmed that the N. vitripennis venom encoding genes are almost exclusively expressed in the venom gland relative to the neighbouring ovary tissue. Novel peptides appear to perform key roles in N. vitripennis venom function as only four of the highest 15 expressed venom encoding genes are bioinformatically annotationed. The high throughput sequencing data also provided evidence for the existence of an additional 471 novel genes in the Nasonia genome that are expressed in the venom gland and ovary. Finally, metagenomic analysis of venom gland transcripts identified viral transcripts that may play an important part in the N. vitripennis venom function. Conclusions The expression level information provided here for the 79 venom encoding genes provides an unbiased dataset that can be used by the N. vitripennis community to identify high value candidates for further functional characterisation. These candidates represent bioactive peptides that have value in drug development pipelines.
2016-12-31 | GSE76257 | GEO
Project description:For many behaviours studied at the phenotypic level, we have little or no idea of where to start searching for “candidate” genes: the transcriptome provides such a starting point. Here we consider transcriptomic changes associated with oviposition in the parasitoid wasp Nasonia vitripennis. Oviposition is a key behaviour, as females are faced with a variety of decisions that will impact offspring fitness. These include choosing between hosts of differing quality, as well as deciding on clutch size and offspring sex ratio. We compared the whole-body transcriptomes of resting or ovipositing female Nasonia using a “DEEP-Sage” gene expression approach on the Illumina sequencing platform. Single 2-day old mated Nasonia vitripennis females (ASymC strain) were isolated in a glass vial and provided with a single host to produce F1 daughters. Eight 2-day old mated F1 females were subsequently provided with three hosts to produce the F2 test females. We randomly selected one host from each F1 female, and isolated 16 2-day old mated F2 test females in glass tubes, of which eight were randomly allocated to the oviposition treatment and eight to the resting treatment. We provided the test females with a single host for 24 hours as pre-treatment to facilitate egg development. We then discarded the pre-treatment hosts and gave each female a piece of chromatography paper soaked in honey solution for a further 24 hours. For the experiment, we transferred the females to 1.5 mL Eppendorf tubes that contained a single host for the oviposition experiment, or were empty for the resting treatment. After 60 mins, females were flash-frozen in liquid nitrogen and stored on dry-ice until the addition of RNAlater-ICE (Ambion, Austin, TX, USA) after which they were transferred to -20C. All females in the oviposition treatment were observed to have commenced ovipositing. We pooled the F2 test females from each F1 mother according to treatment, generating a total of eight pooled samples per treatment (consisting of 8 females per pool) for RNA isolation and sequencing.
2013-07-22 | E-GEOD-43352 | biostudies-arrayexpress
Project description:RNA from Developmental analysis of DNA Methylation in the Parasitoid Wasp Nasonia vitripennis
| PRJEB83044 | ENA