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XbGigChil

ABSTRACT: Gigantidas childressi

PROVIDER: PRJEB83485 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ERR14075531_1.fastq.gz	Fastqsanger.gz
	ERR14075531_2.fastq.gz	Fastqsanger.gz
	ERR14075532_1.fastq.gz	Fastqsanger.gz
	ERR14075532_2.fastq.gz	Fastqsanger.gz

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Project description:“Bathymodiolus” childressi is a species of deep-sea mussels found predominantly in the Gulf of Mexico harbouring a methane oxidizing symbiont (MOX). Ca. Endonucleobacter is a gammaproteobacterial intranuclear parasite that infects the ciliated edge cells of the deep-sea mussel “B.” childressi. After a single Ca. Endonucleobacter cell invades the host nucleus, it proliferates massively, increasing the volume of the nucleus up to 50 fold. It is hypothesized that intranuclear parasites use host chromatin as a nutritional source, however, this would lead to a destabilization of the replication niche. The aim of this study was to investigate how Ca. Endonucleobacter thrives in the nucleus and how it affects the host cell using various “-omics' ' approaches. In addition to protein identification we generated a high quality genome draft for Ca. Endonucleobacter using a hybrid assembly pipeline (PacBio + Illumina reads). We annotated this high quality genome draft and used it as a reference to identify the proteins responsible for the molecular interaction between Ca. Endonucleobacter and its host cell. The 12 specimens of “B.” childressi analyzed in this study were collected during five dives from the Mississippi Canyon site (MC853, 28º07’ N; -089º08’ W) and the Green Canyon site (GC234, 27º45’ N; -091º13’ W) at a water depth of 1,070 and 540 m, respectively. The mussels were selected according to their degree of Ca. Endonucleobacter infection. According to this criterion, the “B.” childressi specimens were divided into two conditions: 8 were classified as “infected”, while 4 were classified as “non-infected” (negative control). For each specimen, we prepare microdissected ciliated edge samples in triplicates.

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