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Respiratory microbiota and ventilator-associated pneumonia in children

ABSTRACT: Respiratory microbiota and ventilator-associated pneumonia in children

PROVIDER: PRJEB83626 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR14082800_1.fastq.gz	Fastqsanger.gz
	ERR14082800_2.fastq.gz	Fastqsanger.gz
	ERR14082801_1.fastq.gz	Fastqsanger.gz
	ERR14082801_2.fastq.gz	Fastqsanger.gz
	ERR14082802_1.fastq.gz	Fastqsanger.gz

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Systemic gene expression profiles in patients with ventilator-associated pneumonia

Project description:This project was a prospective translational study aimed at evaluating gene expression profiles (GEP) of patients with ventilator-associated pneumonia (VAP) . GEP of VAP were compared with a control group of patients which did not developed ventilator-associated lower respiratory tract infection despite being subjected to mechanical ventilation.

2018-01-31 | E-MTAB-5638 | biostudies-arrayexpress

Differential gene expression between critically ill trauma patients who do and do not go on to develop ventilator-associated pneumonia

Project description:Objective: Identify genes that are differentially expressed between critically ill trauma patients who go on to develop ventilator-associated pneumonia (VAP) compared to similar patients who do not develop VAP Using gene expression differences, develop a model that predicts which patients are at greater risk of developing VAP. Prospective observational study, analysis of gene expression in 20 patient samples, 10 that developed ventilator-associated pneumonia, 10 that did not

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Project description:Ventilator associated pneumonia (VAP) is the 2nd most common hospital acquired infection associated with high morbidity, mortality and increased hospitalization. Current practice of diagnosis is based on clinical symptoms and bronchoalvolar lavage (BAL) culture. The procedures of BAL collections are invasive whereas, endotracheal aspirate (ETA), a matrix of upper airway collection is minimally invasive and underexplored in VAP diagnosis. The study describes first in detail characterization of proteome of longitudinal ETA collections from 16 intubated patients including 11 VAP patients and explores potential utility of ETA in VAP diagnosis.

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Developing a gene expression model for predicting ventilator-associated pneumonia in trauma patients: a pilot study.

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Intubated patients developing Tracheobronchitis or Pneumonia have distinctive complement system gene expression signatures

Project description:The onset of an infection-specific transcriptional program precedes the clinical diagnosis in patients who developed Ventilator-associated pneumonia (VAP). Ventilator-associated tracheobronchitis (VAT) is another respiratory infection affecting<br><br>outcomes in intubated patients, but interactions between VAT and VAP remains unknown.

2011-11-19 | E-MEXP-3001 | biostudies-arrayexpress

Untargeted Blood Lipidomics Analysis in Critically Ill Pediatric Patients with Ventilator-Associated Pneumonia

Project description:Blood samples were collected from 20 patients with suspected ventilator-associated pneumonia (VAP), aged 6 months to 15 years, at days 1, 3, 6, and 12, followed by an untargeted lipidomics analysis using Ultra-High Pressure Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UPLC-HRMS) on a TIMS-TOF/MS platform. Patients were stratified into high (mCPIS > 6, n = 12) and low (mCPIS < 6, n = 8) VAP suspicion groups based on the modified pediatric clinical pulmonary index score (mCPIS).

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Late-onset ventilator-associated pneumonia due to Mycobacterium chelonae

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Identification of respiratory microbiota markers in ventilator-associated pneumonia.

Project description:PurposeTo compare bacteria recovered by standard cultures and metataxonomics, particularly with regard to ventilator-associated pneumonia (VAP) pathogens, and to determine if the presence of particular bacteria or microbiota in tracheal and oropharyngeal secretions during the course of intubation was associated with the development of VAP.MethodsIn this case-control study, oropharyngeal secretions and endotracheal aspirate were collected daily in mechanically ventilated patients. Culture and metataxonomics (16S rRNA gene-based taxonomic profiling of bacterial communities) were performed on serial upper respiratory samples from patients with late-onset definite VAP and their respective controls.ResultsMetataxonomic analyses showed that a low relative abundance of Bacilli at the time of intubation in the oropharyngeal secretions was strongly associated with the subsequent development of VAP. On the day of VAP, the quantity of human and bacterial DNA in both tracheal and oropharyngeal secretions was significantly higher in patients with VAP than in matched controls with similar ventilation times. Molecular techniques identified the pathogen(s) of VAP found by culture, but also many more bacteria, classically difficult to culture, such as Mycoplasma spp. and anaerobes.ConclusionsMolecular analyses of respiratory specimens identified markers associated with the development of VAP, as well as important differences in the taxa abundance between VAP and controls. Further prospective trials are needed to test the predictive value of these markers, as well as the relevance of uncultured bacteria in the pathogenesis of VAP.

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Headspace volatile organic compounds from bacteria implicated in ventilator-associated pneumonia analysed by TD-GC/MS

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2018-08-08 | MTBLS497 | MetaboLights

Nasopharyngeal microbiota, host transcriptome and disease severity in children with respiratory syncytial virus infection

Project description:Rationale: Respiratory syncytial virus (RSV) is the leading cause of acute lower respiratory tract infections and hospitalizations in infants worldwide. Known risk factors, however, incompletely explain the variability of RSV disease severity among children. We postulate that severity of RSV infection is influenced in part by modulation of the host immune response by the local microbial ecosystem at the time of infection. Objectives: To define whether different nasopharyngeal microbiota profiles are associated with distinct host transcriptome profiles and severity in children with RSV infection. Methods: We analyzed the nasopharyngeal microbiota profiles of young children with mild and severe RSV disease and healthy matched controls by 16S-rRNA sequencing. In parallel, we analyzed whole blood gene expression profiles to study the relationship between microbial community composition, the RSV-induced host transcriptional response and clinical disease severity. Measurements and Main results: We identified five nasopharyngeal microbiota profiles characterized by enrichment of H. influenzae, Streptococcus, Corynebacterium, Moraxella or S. aureus. RSV infection and RSV hospitalization were positively associated with H. influenzae and Streptococcus, and negatively associated with S. aureus abundance, independent of age. The host response to RSV was defined by overexpression of interferon-related genes, and this was independent of the microbiota composition. On the other hand, transcriptome profiles of RSV infected children with H. influenzae and Streptococcus-dominated microbiota were characterized by greater overexpression of genes linked to toll-like receptor-signaling and neutrophil activation and were more frequently hospitalized Conclusions: Our data suggest an immunomodulatory role for the resident nasopharyngeal microbial community early in RSV infection, potentially affecting RSV disease severity.

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