Slaughterhouse microbiome analysis Spain
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB83990 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Copepod microbiome method comparison
| PRJNA698385 | ENA
Project description:Coral Microbiome Preservation and Extraction Method Comparison
| PRJNA544686 | ENA
Project description:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria, such as Bacillus cereus, to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of B. cereus ATCC 14579 exposed to four different disinfectants (i.e., benzalkonium chloride, sodium hypochlorite, hydrogen peroxide, and peracetic acid) were analyzed. The physiological response of B. cereus to different concentrations of the disinfectants used was investigated. For each disinfectant, concentrations leading to the attenuation of growth, growth arrest, and cell death were studied in more detail. The simultaneous analysis of the transcriptional responses of B. cereus upon exposure to the different concentrations of disinfectants revealed common responses induced by the four disinfectants. Notably, genes involved in the general and oxidative stress responses were commonly up-regulated. Furthermore, the obtained results indicate that all the disinfectants also induce specific responses. Exposure to benzalkonium chloride, a disinfectant known to induce membrane damage, specifically induced genes involved in the fatty acid metabolism. Benzalkonium chloride induced-membrane damage was confirmed by fluorescence microscopy and fatty acid analysis confirmed that fatty acid composition of cell membrane was affected upon exposure to benzalkonium chloride. Sodium hypochlorite induced genes involved in sulfur and sulfur-containing amino acids metabolism, which correlated with the observed sodium hypochlorite-specific induction of oxidation of sulphydryl groups. Hydrogen peroxide and peracetic acid exposures induced genes involved in DNA damage and the SOS response. Notably, hydrogen peroxide and peracetic acid-treated cells exhibited higher mutation rates corroborating with the induced SOS response. Understanding the mechanisms displayed by microorganisms coping with disinfectants-induced stress may allow for design of more efficient sequential and/or disinfectant combination treatments in food processing environments.
2010-03-31 | GSE18807 | GEO
Project description:Comparison of disinfectants on water-derived biofilms.
| PRJNA614901 | ENA
Project description:The goals of this study are to use SWATH-MS to detect bacterial proteomic profiles of wild-type Acinetobacter baylyi ADP1, and its protein response under the exposure of disinfectants, including chloramine and free chlorine. The concentrations of disinfectants were 10 mg/L. The group without dosing disinfectant was the control group. Each concentration was conducted in triplicate. By comparing the proteomic profiles of experimental groups and control group, the effects of disinfectants on translational levels can be revealed.
2021-09-09 | PXD016794 | Pride
Project description:Comparison of hexachlorocyclohexane (HCH) contaminated soils from Spain with a community-specific microarray. These results are being submitted for publication and represent the first use of microarrays for analysis of soil DNA and the first community-specific microarray design. Keywords: other
2004-11-19 | GSE1985 | GEO
Project description:Grapevine microbiome (La Rioja, Spain) Raw sequence reads
| PRJNA1337338 | ENA