Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| ERR14242266_1.fastq.gz | Fastqsanger.gz | |||
| ERR14242266_2.fastq.gz | Fastqsanger.gz | |||
| ERR14231568.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:Sex determination is the process by which and original bipotential gonad differentiate into either a testis or ovaries. While mammals and birds determine their sex solely by genetic clues (genetic sex determination, GSD), other vertebrates like the turtle Trachemys scripta are influenced by environmental factors, like temperature (environmental sex determination, ESD). In both cases an initially bipotential gonad develops into either testes or ovaries in response to GSD or ESD cues. In order to shed light into the differences and similarities between sex determination systems we performed single-cell RNA-seq on Trachemys scripta developing gonads during the sex determination window.
2024-12-30 | GSE271230 | GEO
Project description:Myelin is one of the most important features of the vertebrate nervous system, formed by glial cells. In the peripheral nervous system, Schwann cells produce myelin. While much is known about the molecules and processes involved in mammalian myelination, little is known about it in other vertebrate groups. In this study, we examined myelin development in the peripheral nerves of the turtle Trachemys scripta using qPCR, RNA sequencing, immunofluorescence, and TEM. Our findings indicate that in T. scripta, myelination begins during the late stages of embryonic development and continues beyond hatching. Expression profiles reveal both conservation and divergence of core myelination components between mammals and amphibians; however, direct gene-level comparisons across species require further investigation given our small sample size. The upregulation of orthologous myelin genes in the turtle PNS supports the idea that these components are conserved, although their timing, regulation, or network structure may differ across tetrapods. We based our inference of conservation on orthology and the coordinated expression of key myelin genes (e.g., ErbB2, Mag, Mpz, Mbp, Pmp22) in T. scripta. TEM analysis of turtle sciatic nerves shows myelin rings in a few axons starting at stage 21, increasing significantly by stage 24, and becoming prominent in most axons of the adult nerve. Furthermore, antibodies against MPZ, DRP2, and Kv1.1 indicate that T. scripta adult peripheral nerves at various axial levels contain myelin segments with structures consistent with appositions and Schmidt-Lanterman incisures. Overall, this is the first study of PNS myelin development in a reptile, demonstrating that myelination is a highly conserved process in vertebrates.
2026-06-04 | GSE334447 | GEO