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FMD Virus RT-PCR Control Samples

ABSTRACT: This study involved utilization of a DNA Plasmid that contained a full-length FMDV genome. The DNA plasmid was grown in bacteria before ultra-deep sequencing on a Illumina GAII. The Plasmid was then PCR amplified and again sequenced on a GAII, to asses the impact of PCR on the amount of observed mutations in the sequence data. The Plasmid was then transcribed, then reverse transcribed and PCR amplified before sequenced on a GAII, to asses the impact of RT on the amount of observed mutations in the sequence data. These control samples essentially allow the impact of sample processing on sequencing errors to be assessed in terms of viral quasispecies analyses.

PROVIDER: PRJEB8601 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR776655.fastq.gz	Fastqsanger.gz
	ERR776656.fastq.gz	Fastqsanger.gz
	ERR776657.fastq.gz	Fastqsanger.gz
	ERR776658.fastq.gz	Fastqsanger.gz

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