Project description:Most individuals infected with Mycobacterium tuberculosis can control the infection by forming and maintaining TB granulomas at the local infection foci. However, when the chronic infection (also known as latency) becomes active, the caseous center of TB granuloma enlarges, and it liquefies and cavitates, ultimately releasing bacilli into airway. Deciphering how genes are regulated within TB granulomas will help to understand the granuloma biology. Therefore, we performed genome-wide microarray on caseous human pulmonary TB granulomas and compared with normal lung tissues.
Project description:Pulmonary tuberculosis (TB) generates chronic systemic inflammation and metabolic dysregulation. The liver is the master regulator of metabolism and to determine the impact of pulmonary TB on this organ we undertook unbiased mRNA analyses of the liver in mice with TB. Pulmonary TB led to upregulation of genes in the liver related to interferon signalling and glycolysis, and downregulation of genes encoding gluconeogenesis rate-limiting enzyme
Project description:To evaluate whether TB infections are associated with any lncRNA signatures in humans, we therefore used human lncRNAs microarray and hierarchical clustering analyses to compare lncRNAs expression in active TB patients and healthy controls. From 15,683 denoted lncRNAs, 5076 lncRNAs were identified to be differentially expressed (TB/HC > 2 or TB/HC< 0.5) in peripheral blood mononuclear cells (PBMCs) between TB and healthy subjects.
