Project description:PARTIAL UPLOAD: Metaproteomic dataset pertaining to marine biofilms analysed after growth on low-density polyethylene (LDPE) for 3 (D3) and 7 (D7) days. Four replicates per condition.
Project description:The aim of this study was to investigate the plastic colonisation process, to identify the active taxa involved in biofilm formation and the mechanisms used to initiate colonisation. To achieve this, a marine plastisphere characterised by active hydrocarbonoclastic genera was used as the inoculum for a short-term microcosm experiment using virgin low-density polyethylene as the sole carbon source. Following incubation for 1 and 2 weeks (representing early and late colonisation, respectively), a taxonomic and comparative metaproteomic approach was used to explore shifts in diversity and function.
Project description:To characterize the taxonomic and functional diversity of biofilms on plastics in marine environments, plastic pellets (PE and PS, ø 3mm) and wooden pellets (as organic control) were incubated at three stations: at the Baltic Sea coast in Heiligendamm (coast), in a dead branch of the river Warnow in Warnemünde (inlet), and in the Warnow estuary (estuary). After two weeks of incubation, all pellets were frozen for subsequent metagenome sequencing and metaproteomic analysis. Biofilm communities in the samples were compared on multiple levels: a) between the two plastic materials, b) between the individual incubation sites, and c) between the plastic materials and the wooden control. Using a semiquantitative approach, we established metaproteome profiles, which reflect the dominant taxonomic groups as well as abundant metabolic functions in the respective samples.
Project description:Histone posttranslational modifications are a main driver for gene regulation, nevertheless, several questions remain unanswered regarding their heritability. The replisome recycles parental histones through the cell cycle, but how this is accomplished in vivo and impacts epigenetic inheritance remains unknown. Here, we show that Caenorhabditis elegans mutants with disrupted replisome-mediated histone recycling display transgenerational defects in RNAi inheritance and fertility. These strains uncovered a particular kind of heterochromatic domain that we called Corrie domains, which are located at the borders of relatively high and low H3K9me3-rich regions and contain gene and AT-rich transposable-element (TE) clusters poised for activation upon deficient H3K9me3 maintenance. Corrie domains are conserved in Mus musculus highlighting an evolutionarily conserved heterochromatic architecture that operates as a hub for regulatory diversity.