Project description:Gradient-enriched quiescent cells of a 6 days stationary phase culture were refed with rich medium (Yeast Peptone Dextrose 2%) in the presence or absence of RNAPII inhibition by various means (rpb1-1 thermosensitive mutant, thiolutin). Samples were harvested in quiescence and 30 minutes post-refeeding.
Project description:The main objective of this project is the characterization of cell-to-cell transcriptional heterogeneity in and out of the yeast quiescent state. Do quiescent cells manage to respond rapidly to their environment through a general transcriptional permissiveness, or through a cell- to-cell heterogeneity in the transcriptional response?
Project description:Saccharomyces cerevisiae is an excellent microorganism for industrial succinic acid production, but high succinic acid concentration will inhibit the growth of Saccharomyces cerevisiae then reduce the production of succinic acid. Through analysis the transcriptomic data of Saccharomyces cerevisiae with different genetic backgrounds under different succinic acid stress, we hope to find the response mechanism of Saccharomyces cerevisiae to succinic acid.
Project description:LPS was used as a stressor to stimulate the model organism Saccharomyces cerevisiae. To detect extracellular metabolic information of VOCs. To provide a molecular basis for cellular metabolism of VOCs by proteome.
Project description:Industrial bioethanol production may involve a low pH environment,improving the tolerance of S. cerevisiae to a low pH environment caused by inorganic acids may be of industrial importance to control bacterial contamination, increase ethanol yield and reduce production cost. Through analysis the transcriptomic data of Saccharomyces cerevisiae with different ploidy under low pH stress, we hope to find the tolerance mechanism of Saccharomyces cerevisiae to low pH.