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Ninja-seq

ABSTRACT: ninja-seq

PROVIDER: PRJEB88528 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR14933512.fastq.gz	Fastqsanger.gz
	ERR14933513.fastq.gz	Fastqsanger.gz
	ERR14933514.fastq.gz	Fastqsanger.gz
	ERR14933515.fastq.gz	Fastqsanger.gz
	ERR14997955.fastq.gz	Fastqsanger.gz

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Root transcriptome of ninja-1

2015-05-28 | GSE65840 | GEO

Root transcriptome of ninja-1

Project description:A major role of NINJA is to repress root jasmonate signalling and allow normal cell elongation. To further characterize the transcriptional changes occurring in the root in the absence of a NINJA-dependent repression mechanism, we conducted a microarray analysis of ninja-1 versus WT roots. Root transcriptome of Col-0 (WT) versus ninja-1, both carrying the JAZ10pro-GUSPlus (JGP) reporter, grown in basal aseptic conditions. Biological replicates: 3 for the WT control and 3 for the ninja-1 mutant.

2015-05-28 | E-GEOD-65840 | biostudies-arrayexpress

NINJA connects JAZ proteins to the co-repressor TOPLESS

Project description:NINJA (At4g28910) and TOPLESS proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress- and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants. Two-condition experiment, Col-0 vs. plants silencing NINJA (RNAi-NINJA) and both Col-0 vs RNAi NINJA treated with coronatine . Biological replicates: 4 control replicates untreated , 4 RNAi NINJA untreated replicates, 4 control treated replicates and 4 RNAi NINJA treated replicates

2010-04-05 | E-GEOD-19455 | biostudies-arrayexpress

RNA-seq analysis of frs7;frs12;ninja-1 triple loss-of-function seedlings

Project description:Gene expression analysis of Arabidopsis thaliana frs7;frs12;ninja-1triple mutant line in comparison with control lines (frs7;frs12, ninja-1 and Col0).

2020-04-27 | E-MTAB-7402 | biostudies-arrayexpress

NINJA connects JAZ proteins to the co-repressor TOPLESS

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RNA-seq analysis of frs7 frs12 ninja-1 triple loss-of-function seedlings

Project description:RNA-seq analysis of frs7 frs12 ninja-1 triple loss-of-function seedlings

| PRJEB29717 | ENA

Grad-seq Enterococcus faecalis and faecium

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Grad-seq Clostridium difficile 630

Project description:Grad-seq in Clostridium difficile 630. Cell lysate is analyzed in a gradient and fractionated into 21 fractions which are analysed for proteins by MS and for transcripts by RNA-sequencing.

2021-05-12 | PXD022525 | Pride

Role of NINJA in root jasmonate signaling.

Project description:Wound responses in plants have to be coordinated between organs so that locally reduced growth in a wounded tissue is balanced by appropriate growth elsewhere in the body. We used a JASMONATE ZIM DOMAIN 10 (JAZ10) reporter to screen for mutants affected in the organ-specific activation of jasmonate (JA) signaling in Arabidopsis thaliana seedlings. Wounding one cotyledon activated the reporter in both aerial and root tissues, and this was either disrupted or restricted to certain organs in mutant alleles of core components of the JA pathway including COI1, OPR3, and JAR1. In contrast, three other mutants showed constitutive activation of the reporter in the roots and hypocotyls of unwounded seedlings. All three lines harbored mutations in Novel Interactor of JAZ (NINJA), which encodes part of a repressor complex that negatively regulates JA signaling. These ninja mutants displayed shorter roots mimicking JA-mediated growth inhibition, and this was due to reduced cell elongation. Remarkably, this phenotype and the constitutive JAZ10 expression were still observed in backgrounds lacking the ability to synthesize JA or the key transcriptional activator MYC2. Therefore, JA-like responses can be recapitulated in specific tissues without changing a plant's ability to make or perceive JA, and MYC2 either has no role or is not the only derepressed transcription factor in ninja mutants. Our results show that the role of NINJA in the root is to repress JA signaling and allow normal cell elongation. Furthermore, the regulation of the JA pathway differs between roots and aerial tissues at all levels, from JA biosynthesis to transcriptional activation.

| S-EPMC3780868 | biostudies-literature

Rhizogenic Agrobacterium protein RolB interacts with the TOPLESS and NINJA repressor proteins to reprogram plant jasmonate signaling.

Project description:Rhizogenic Agrobacterium protein RolB interacts with the TOPLESS and NINJA repressor proteins to reprogram plant jasmonate signaling.

| PRJEB57826 | ENA

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