Project description:Analysis of genome-wide hydroxymethylation within infant placenta tissue collected at term. These samples have been collected from the Rhode Island Child Health Study (RICHS) cohort.
Project description:Genome-wide profiling of placental DNA methylation in relation to neurobehavioral development. The Illumina 450k methylation array was used to profile 335 samples. These samples have been collected from the Rhode Island Child Health Study (RICHS).
Project description:Human blood from 12 donors was exposed to increasing doses of neutron radiation at two sites: the Rhode Island Nuclear Science Center (RINSC), and the Radiological Research Accelerator Facility (RARAF) at Columbia University. The exposure at the Rhode Island Nuclear Science Center was largely thermal neutrons, and the exposure at the Radiological Research Accelerator Facility was largely fast neutrons. Samples were measured for RNASeq differential expression.
Project description:Genome-wide profiling of placental DNA methylation in relation to neurobehavioral development. The Illumina 450k methylation array was used to profile 335 samples. These samples have been collected from the Rhode Island Child Health Study (RICHS). Illumina analysis performed on 335 human placentas
Project description:Chromosomal segmental copy number variation (CNV) has been recently recognized as a very important source of genetic variability. Some CNV loci involve genes or conserved regulatory regions. Compelling evidence indicates that CNVs impact genome functions. The chicken is a very important farm animal species which has also served as model animal for biological and biomedical research for hundreds of years. A map of CNVs in chickens could facilitate the identification of chromosome regions that segregate for important agricultural and disease phenotypes. NimbleGen 385k whole genome tiling arrays were used to map CNVs in the chicken. This study has identified 96 CNVs in three lines of chickens (broiler, Leghorn and Rhode Island red). These CNVs encompass 16 Mb (1.3%) of the chicken genome. Twenty six CNVs were found in two or more animals. Smaller sized CNVs mostly affect none coding sequences while larger CNV regions involve genes, for example prolactin receptor, aldose reductase and zinc finger proteins, suggesting chicken CNVs potentially affect agricultural or disease related traits.
Project description:Copy number variation (CNV) is important and widespread in the genome, and is a major cause of disease and phenotypic diversity. Herein, we perform a genome-wide analysis of CNVs in the 12 diversified chicken genomes based on next-generation sequencing. We apply aCGH experiments to confirm our predicted CNVs. Results from aCGH agree well with our findings and the Pearson’s correlation values between the test and reference samples range from 0.644 to 0.722. The whole blood samples from Cornish, Rhode Island Red, and White Leghorn
Project description:One ONT-ULK sequencing run from the kidney of a single male CDMR (Bathyergus suillus) sample used to make the mBatSui1.1.primary genome assembly as an evolutionary comparator to our telomere-to-telomere naked mole-rat genome assembly. Specifically, we assembled a CDMR from a wild-derived sample in South African cape and sequenced in Toronto, Canada, using PacBio HiFi (89 Gb, read N50 = 18 Kb) and ONT-ULK (55 Gb, read N50 = 43 Kb) reads (contig N50 = 33 Mb, Compleasm S = 99%, QV = 71.0). This accession stores the ONT-ULK data for this assembly.
