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OdWebBurs1.metagenome

ABSTRACT: Weberella bursa sponge metagenome assembly, odWebBurs1.metagenome

PROVIDER: PRJEB88817 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	odWebBurs1.Akkermansiaceae_1.1.primary.fa.gz	Other
	odWebBurs1.Arenicellales_1.1.primary.fa.gz	Other
	odWebBurs1.Arenicellales_2.1.primary.fa.gz	Other
	odWebBurs1.Bdellovibrionales_1.1.primary.fa.gz	Other

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Project description:Metagenome data from soil samples were collected at 0 to 10cm deep from 2 avocado orchards in Channybearup, Western Australia, in 2024. Amplicon sequence variant (ASV) tables were constructed based on the DADA2 pipeline with default parameters.

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Oceanographic setting influences the prokaryotic community and metabolome in deep-sea sponges

Project description:Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many of these compounds. Yet, environmental influences on the sponge-associated microbiota and bioactive compound production remain elusive. Here, we investigated the changes of microbiota and metabolomes in sponges along a depth range of 1232 m. Using 16S rRNA gene amplicon sequencing and untargeted metabolomics, we assessed prokaryotic and chemical diversities in three deep-sea sponge species: Geodia barretti, Stryphnus fortis, and Weberella bursa. Both prokaryotic communities and metabolome varied significantly with depth, which we hypothesized to be the effect of different water masses. Up to 35.5% of microbial ASVs (amplicon sequence variants) showed significant changes with depth while phylum-level composition of host microbiome remained unchanged. The metabolome varied with depth, with relative quantities of known bioactive compounds increasing or decreasing strongly. Other metabolites varying with depth were compatible solutes regulating osmolarity of the cells. Correlations between prokaryotic community and the bioactive compounds in G. barretti suggested members of Acidobacteria, Proteobacteria, Chloroflexi, or an unclassified prokaryote as potential producers.

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Project description:Agelas tubulata (tubulate sponge) sponge metagenome assembly, odAgeTubu1.metagenome

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