Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IdOpoFlor

ABSTRACT: Opomyza florum

PROVIDER: PRJEB88860 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR14886553.fastq.gz	Fastqsanger.gz
	ERR14895966_1.fastq.gz	Fastqsanger.gz
	ERR14895966_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Opomyza florum

Project description:Opomyza florum overview

| PRJNA1453675 | ENA

idOpoFlor2

Project description:Opomyza florum genome assembly, idOpoFlor2

| PRJEB111437 | ENA

idOpoFlor

Project description:Opomyza florum, genomic and transcriptomic data

| PRJEB88859 | ENA

idOpoFlor2 alternate haplotype

Project description:Opomyza florum genome assembly, idOpoFlor2, alternate haplotype

| PRJEB111438 | ENA

Transcriptome profiling of Mesoplasma florum strain L1 using RNA sequencing (RNA-Seq) and genome-wide 5'-rapid amplification of cDNA ends (5'-RACE)

Project description:Mesoplasma florum is a near-minimal bacterium belonging to the Mollicutes class particularly characterized by its small genome, fast growth rate, and lack of pathogenic potential. Here, we report the first genome-wide transcriptomic analysis of this microorganism using directional RNA sequencing (RNA-Seq) and 5'-rapid amplification of cDNA ends (5'-RACE). 5'-RACE sequencing reads were used to identify 605 putative transcription start sites (TSSs), of which more than 400 could be associated to a conserved promoter sequence. Along with terminator predictions, these TSSs allowed the reconstruction of M. florum transcription units, covering more than 90% of annotated genes. We evaluated the expression level of all M. florum protein coding genes using paired-end RNA-Seq reads according to the number of fragments per kilobase per million of mapped reads (FPKM). FPKM values were converted to absolute transcript abundances using biomass quantification data. These efforts aim at reaching a deep understanding of global cellular mechanisms in M. florum and will guide future genome engineering projects in this simple organism.

2020-11-02 | GSE152985 | GEO

Mesoplasma florum proteome analysis by 2D LC-MS/MS

Project description:The near-minimal bacterium Mesoplasma florum constitutes an interesting model for synthetic genomics and systems biology studies due to its small genome, fast growth rate, and lack of pathogenic potential. However, some fundamental aspects of its biology remain largely unexplored. Here, we report a broad yet remarkably detailed characterization of M. florum by combining a wide variety of experimental approaches. More specifically, we investigated several physical and physiological aspects of this bacterium, and performed the first genome-wide analysis of its transcriptome and proteome using RNA sequencing techniques and two-dimensional liquid chromatography-tandem mass spectrometry. These analyses notably revealed the M. florum consensus promoter, a first experimental cartography of transcription units, as well as the transcription and expression levels of all annotated genes. We also converted gene transcription and expression levels into absolute molecular abundances using biomass quantification results, generating an unprecedented view of the M. florum cellular composition and functions. These characterization efforts will provide an experimental foundation for the development of a genome-scale metabolic model for M. florum and will guide future genome engineering endeavours in this simple organism.

2020-11-16 | PXD019922 | Pride

idOpoGerm

Project description:Opomyza germinationis

| PRJEB79818 | ENA

Opomyza germinationis

Project description:Opomyza germinationis overview

| PRJNA1306273 | ENA

Fructilactobacillus florum

Project description:Lactobacillus florum overview

| PRJNA172936 | ENA

Mesoplasma florum W37

Project description:Mesoplasma florum W37 Genome sequencing