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Genome-wide study of the MSL1 effect on gene expression in Drosophila melanogaster

ABSTRACT: Genome-wide study of the MSL1 effect on gene expression in Drosophila melanogaster

PROVIDER: PRJEB8928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR414112_1.fastq.gz	Fastqsanger.gz
	ERR414112_2.fastq.gz	Fastqsanger.gz
	ERR414116_1.fastq.gz	Fastqsanger.gz
	ERR414116_2.fastq.gz	Fastqsanger.gz
	ERR414121_1.fastq.gz	Fastqsanger.gz

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Genome-wide study of the MSL1 effect on gene expression in Drosophila melanogaster

Project description:Measurement of gene expression by strand specific RNA-Seq in male (S2) Drosophila melanogaster cells upon depletion of MSL1 by dsRNA compared to the eGFP RNAi control.

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Genome-wide study of the MSL1 effect on gene expression and mRNA capping efficiency

Project description:Measurement of capping efficiency (by 5'CAP and 5'noCAP sequencing) in male (S2) and female (Kc) Drosophila melanogaster cells upon depletion of MSL1 by dsRNA compared to the eGFP RNAi control. The measurement of capping efficiency was combined with gene expression measurement by strand specific RNA-Seq in female (Kc) Drosophila melanogaster cells

2023-09-30 | E-MTAB-2235 | biostudies-arrayexpress

ChIP-seq of MSL1 in male and female fly samples

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N-terminus of Drosophila MSL1 is critical for dosage compensation

Project description:The male-specific dosage compensation complex (DCC), which consists of five proteins and two non-coding roX RNAs, is necessary for the transcriptional enhancement of X-linked genes to compensate for the sex chromosome monosomy in Drosophila XY males, compared with XX females. The MSL1 and MSL2 proteins form the heterotetrameric core of DCC and are critical for the specific recruitment of the DCC to the high-affinity “entry” sites (HAS) on the X chromosome. Here we demonstrated that the N-terminal region of MSL1 is critical for its stability and functions. Amino acid deletions and substitutions in the N-terminal region of MSL1 strongly affect both interaction with roX2 RNA and DCC binding to HAS on the X chromosome. In particular, substitution of the conserved N-terminal amino-acids 3-7 in MSL1GS has an affect on dosage compensation similar to inactivation of genes encoding roX RNAs. MSL1GS binds to promoters like MSL1WT but does not co-bind with MSL2 and MSL3 to X chromosomal HAS. However, over-expression of MSL2 partially restores the functional activity of MSL1GS in dosage compensation. Thus, the interaction of MSL1 with roX RNA is critical for the efficient assembly of DCCs on HAS of the male X chromosome.

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Genome wide distribution of dH1K27me2 in Wild Type Drosophila melanogaster S2 cells

Project description:Genome wide distribution of dH1K27me2 in Wild Type Drosophila melanogaster S2 cells

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Chromatin immunoprecipitation to determine MSL1, MSL3, MOF and H4K16Ac binding in Drosophila SL2 and Kc cells reveals MOF as a key regulator of dosage compensation and gene expression in Drosophila

Project description:Genome-wide binding profiles of MSL1, MSL3, MOF and H4K16Ac in Drosophila SL2 and Kc cells.

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A SUPERFAMILY-WIDE ACTIVITY ATLAS OF SERINE HYDROLASES IN DROSOPHILA MELANOGASTER

Project description:The serine hydrolase (SH) superfamily is perhaps, one of the largest functional enzyme classes in all forms of life, and consists of proteases/peptidases, lipases, and carboxylesterases as representative members. Consistent with the name of this superfamily, all members, without any exception to date, use a nucleophilic serine residue in the enzyme active site to perform hydrolytic-type reactions via a two-step ping-pong mechanism involving a covalent enzyme intermediate. Given the highly conserved catalytic mechanism, this superfamily has served as a classical prototype in the development of several platforms of the chemical proteomics technique, activity-based protein profiling (ABPP), to globally interrogate the functions of its different members in various native, yet complex, biological settings. While ABPP-based proteome-wide activity atlas’ for SH activities are available in numerous organisms, including humans, to the best of our knowledge, such an analysis for this superfamily is lacking in any insect model. To address this, here, we first report a bioinformatics analysis towards the identification and classification of non-redundant SHs in Drosophila melanogaster. Following up on this in silico analysis, leveraging discovery chemoproteomics, we identify and globally map the activities of the SH superfamily during various developmental stages and in different adult tissues of Drosophila. Finally, as proof of concept of the utility of this activity atlas, we highlight sexual dimorphism in SH activities across different tissues in adult Drosophila melanogaster, and together, we prospect new research directions, resources and tools that this study can provide to the fly community.

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