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ENVIRONAGE HDM

ABSTRACT: Environage House Dust Microbiota

PROVIDER: PRJEB89387 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR14984600_1.fastq.gz	Fastqsanger.gz
	ERR14984600_2.fastq.gz	Fastqsanger.gz
	ERR14984800_1.fastq.gz	Fastqsanger.gz
	ERR14984800_2.fastq.gz	Fastqsanger.gz
	ERR14984900_1.fastq.gz	Fastqsanger.gz

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Identification and Characterization of HDM D.p. immunogenic RNA

Project description:We examined the sequence information of the gel-extracted and purified RNA bands #1,#2 and #3 derived from House Dust Mite (HDM) D.p. immunogenic RNA. The individula RNA band was utilized for RNA-seq analysis.

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Project description:The very low density lipoprotein receptor (VLDLR) is a multi-ligand receptor that mediates pleiotropic biological processes, such as brain development. In this dataset, we include the expression data obtained from lungs from mice that had been challenged with house dust mite to induce experimental asthma or saline, as a control. These data are used to obtain genes that are differentially expressed in response to VLDLR signaling. We compared wild type and VLDLR knock out mice that had been sensitized and challenged with house dust mite or saline, as a control. There were total 16 samples with four biological relicates in each group.

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Project description:House dust microbiota raw sequence reads

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Transcriptomic analysis in airway epithelial cells in HDM-sensitized STAT3-WT and STAT3-cKO mice

Project description:Purpose: To elucidate the roles of airway epithelial STAT3 in allergic airway inflammation. Method: Doxycycline-induced airway epithelial cells-specific STAT3-deficient mice (STAT3-cKO) and their genetic control mice (STAT3-WT) were sensitized intratracheally twice with house dust mite (HDM) extract or PBS at a 7-day interval. Airway epithelial cells were isolated by a cell sorter 48 h after the last senstization. N=2 each. Results: Using an optimized data analysis workflow, 206 transcripts showed differential expression between HDM-treated STAT3-WT and STAT3-cKO with adjusted p value <0.05.

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