Amps identification
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB89741 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Primary outcome(s): site-specific gene mutation profiles in advanced colorectal cancer
| 2646399 | ecrin-mdr-crc
Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..
| 2612481 | ecrin-mdr-crc
Project description:Intervention type:DRUG
Name of intervention:Huaier
Dose form / Japanese Medical Device Nomenclature:GRANULES
Route of administration / Site of application:ORAL
Dose per administration:20?
g
Dosing frequency / Frequency of use:OTHER, SPECIFY
20g? per day
Planned duration of intervention:3 months to extending if necessary
Intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary
detailes of teratment arms:hepatocellular carcinoma, breast cancer, colorectal cancer and related gastrointestinal cancers, urologic cancers including prostate cancer, pancreas cancer, and lung cancer, etc.
Comparative intervention name:None
Dose form / Japanese Medical Device Nomenclature:
Route of administration / Site of application:
Dose per administration:
Dosing frequency / Frequency of use:
Planned duration of intervention:
Intended dose regimen:
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.
Study Design: Comparative test, None, No, open(masking not used), EXPLORATORY
| 98681 | ecrin-mdr-crc
Project description:Enhancers regulating Th cell specific cytokine gene expression were studied and identified. However, Il9 regulating enhancer was not identified yet. Active enhancers are characterized with P300, STATs binding and active histone modifications such as H3K4me1 and H3K27ac. Based on P300 ChIP seq analysis, we narrowed down enhancer candidates for Il9 gene expression.
2018-10-01 | GSE106404 | GEO
Project description:This study aims to identify genomic contacts with the nucleolus (nucleolar associated domains, NADs). We established a novel methodology that we applied to identify NADs in embryonic stem cells (ESCs). The results revealed unprecedented distinct layers of genome compartmentalization that are distinguished according to gene expression and chromatin states thereby providing novel insights into basic principles of genome organization and its role in gene expression and cell function.
2021-08-11 | GSE150822 | GEO
Project description:Identification of metabolic gene from Spirulina
| PRJEB25013 | ENA
Project description:Bacterial/Fungal Persistence Gene Identification Project
| PRJNA67953 | ENA
Project description:There is clear evidence that clinical genetic counseling for hereditary cancer saves lives and relieves anxiety. Our aim is to identify novel genes that predispose to familial colorectal cancer. We follow the empirical gene discovery paradigm by identifying high-risk families diagnosed with the same cancer in several family members. Such families and samples are identified from the world’s largest biobank of 16,000 familial cancers in Szczecin. For this study we identified 15 colorectal cancer families with several affected family members. Germline DNA from cases and unaffected family members are sequenced genome-wide at the DKFZ core facility, one of Europe’s top sequencing centers. Based on the proof-of-principle study on melanoma, a single family may allow identification of a high-risk variant when the new genome-wide sequencing technology is applied. In that example, the detected variant targeted a transcription factor binding site which turned out to be the most common gene variant in melanoma yet described, with implications to prognosis. The present project aims at cancer prevention in identifying novel high-risk variants for which predictive gene testing can be established.
| EGAS00001005118 | EGA
Project description:Here we characterized a splicesomal sub-complex including the proteins RBM17, U2SURP and CHERP, and found that these proteins physically interact and regulate reciprocal posttranslational stability; individually knocked down, they cause widely overlapping and consistent splicing and gene expression changes of transcripts enriched for RNA processing factors.
2017-12-04 | MSV000081776 | MassIVE
Project description:In plants, microRNAs (miRNAs) play a critical role in post-transcriptional gene regulation and have been shown to control many genes involved in various biological and metabolic processes. Deep sequencing technologies have facilitated identification of species-specific or lowly expressed as well as conserved or highly expressed miRNAs in plants. Strawberry is one of the most economically important fruit throughout the world.Although miRNAs have been extensively studied in the past five years, limited systematic study of miRNAs has been performed on the Fragaria genus. These results show that regulatory miRNAs exist in agronomically important strawberry and may play an important role in strawberry growth, development, and response to disease.
2012-01-04 | GSE34813 | GEO