MiR-34a KO
Ontology highlight
ABSTRACT:
PROVIDER: PRJEB91112 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative transcriptome analysis of Hela cells transfected with hsa-miR-34a,b-5p or c.
2009-05-13 | GSE15957 | GEO
Project description:In order to investigate the role of DDX21 in RNA activation by miR-34a, next-generation sequencing analysis of RNA extracted from miR-34a transfected H1299 and DDX21 knock-out cell line was performed.
2021-10-18 | GSE185946 | GEO
Project description:The goal of this study was to determine the role of miR-34a in regulating chondrocyte transcript profiles. Next Generation Sequencing of total RNA extracted from mouse KO and WT chondrocytes revealed 175 significantly differentially expressed genes (84 up, 91 down) in miR-34a KO chondrocytes compared to WT cells. We are currently validating potential direct targets of miR-34a from our NGS data and performing computational pathway analysis to identify novel pathways regulated by miR-34a.
2021-03-08 | GSE133775 | GEO
Project description:MicroRNAs (miRNAs or miRs) are small, noncoding RNAs that are implicated in the regulation of nearly all biological processes. Global miRNA biogenesis is altered in many cancers and RNA-binding proteins (RBPs) have been shown to play a role in this process, presenting a promising avenue for targeting miRNA dysregulation in disease. miR-34a exhibits tumor-suppressive functions by targeting cell cycle regulators CDK4/6 and anti-apoptotic factor Bcl-2, among other regulatory pathways such as Wnt, TGF-, and Notch signaling. Many cancers show downregulation or loss of miR-34a, and synthetic miR-34a supplementation has been shown to inhibit tumor growth in vivo; however, the post-transcriptional mechanisms by which miR-34a is lost in cancer are not entirely understood. Here, we have used a proteomics-mediated approach to identify Squamous cell carcinoma antigen recognized by T-cells 3 (SART3) as a putative pre-miR-34a-binding protein. SART3 is a spliceosome recycling factor and nuclear RBP with no previously reported role in miRNA regulation. We demonstrate that SART3 binds pre-miR-34a with specificity over pre-let-7d and begin to elucidate a new functional role for this protein in non-small lung cancer cells. Overexpression of SART3 led to increased miR-34a levels, downregulation of the miR-34a target genes CDK4 and CDK6, and cell cycle arrest in the G1 phase. In vitro binding studies showed that the RNA-recognition motifs within the SART3 sequence are responsible for selective pre-miR-34a binding. Collectively, our results present evidence for an influential role of SART3 in miR-34a biogenesis and cell cycle progression.
2019-10-14 | PXD015278 | Pride
Project description:To identify potential targets of miR-34a, we performed transcriptional profiling on proneural TS543 GBM cells, focusing on mRNAs whose levels decreased in response to miR-34a transfection as compared to control oligonucleotide. Proneural TS543 GBM cells were transfected with 100 nM hsa-miR-34a or control oligonucleotide using Hiperfect transfection reagent (Qiagen). After 3 days, RNA was isolated and expression analyses were performed using Illumina HT-12 bead array. The microarray dataset was normalized using a variance stable normalization (VSN) procedure in the ‘lumi’ package from the Bioconductor framework.
2012-03-26 | E-GEOD-34242 | biostudies-arrayexpress
Project description:To identify putative novel specific targets of miR-34a, miR-122, miR-206 and miR-210, we overexpressed these miRNAs in human Hela cells by transfecting them with synthetic pre-miRNAs or a synthetic “negative” pre-miRNA as control (miR-Neg1). RNA samples were harvested at 48 hours post-transfection and 2 independent experiments were carried out.
2021-12-20 | GSE33235 | GEO
Project description:In order to examine the consequences of human miR-34a induction on the transcriptome, HCT116 cells (a colon cancer cell line) were infected with a retrovirus that produces miR-34a. Gene expression profiles were then monitored using Affymetrix microarrays. Affymetrix microarrays were used to examine the transcriptomes of HCT116 cells infected with an empty retroviral vector (pMSCV-PIG) or a retroviral vector that expresses human miR-34a. Keywords: comparison of cells with or without enforced miR-34a expression
2007-06-07 | GSE7754 | GEO
Project description:To investigate the behavior of RNA Pol II during RNA activation by miR-34a,the ChIP-Seq analysis of RNA Poll II in NCI-H1299 cell line was performed. This experiment analyzes how RNA pol II is activated by the introduction of miR-34a in some genes whose transcription is induced by miR-34a. From the analysis of this data, the purpose is to clarify the molecular mechanism of RNA activation by miR-34a.
2021-02-01 | GSE161321 | GEO
Project description:Cell lines of a vGPCR-driven tumor model have been compared to identify KSHV-vGPCR-dependent mechanisms of tumor progression. Cell lines include two biological replicates of a vGPCR-transformed tumor cell line (vGPCR-TC#1+#2) generated by passaging a vGPCR-BALB/c-3T3 cell line (also included) through athymic nude and BALB/c mice. A vGPCR-knockdown tumor cell line (derived from vGPCR-TC#1 generated by lentiviral knockdown) and a GFP-transduced control BALB/c-3T3 cell line are included as well. Data also include a vGPCR-TC#1-derived cell line were miR-34a-5p was knocked down using a Tough Decoy RNA, called TuDmiR-34a-TC#1. Additionally, two miR-34a overexpressing cell lines have been generated derived from the parental vGPCR-3T3 cells and the tumor cell line vGPCR-TC#2 named miR-34a-vGPCR-3T3 respectively miR-34a-vGPCR-TC#2.
2016-02-09 | E-MTAB-3393 | biostudies-arrayexpress
Project description:miR-34a and miR-34b/c genes are frequently epigenetically silenced in primary CRCs. However, the in vivo relevance of miR-34a/b/c for suppression of intestinal tumor formation has not been analyzed by genetic approaches. ApcMin/+ mice with deletion of the miR-34a and miR-34b/c genes were generated and analyzed. The mRNA expression profiles of intestinal adenomas with and without functional miR-34a/b/c genes were compared.
2017-05-28 | GSE84138 | GEO