Metagenomic and Metatranscriptomic Analysis of the Microbiomes Residing in Two-Stage Anaerobic Reactors for Capric Acid Production from Sugarcane Vinasse
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PROVIDER: PRJEB92179 | ENA |
REPOSITORIES: ENA
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Project description:co-fermentation of sugarcane vinasse and glycerol
| PRJEB47724 | ENA
Project description:Reactors applied to the treatment of vinasse - FEAGRI / UNICAMP
| PRJEB35470 | ENA
Project description:Reactors applied to the treatment of vinasse - FEAGRI / UNICAMP
| PRJEB35469 | ENA
Project description:BackgroundThe production of 1 L of ethanol from sugarcane generates up to 12 L of vinasse, which is a liquid waste containing an as-yet uncharacterized microbial assemblage. Most vinasse is destined for use as a fertilizer on the sugarcane fields because of the high organic and K content; however, increased N2O emissions have been observed when vinasse is co-applied with inorganic N fertilizers. Here we aimed to characterize the microbial assemblage of vinasse to determine the gene potential of vinasse microbes for contributing to negative environmental effects during fertirrigation and/or to the obstruction of bioethanol fermentation.ResultsWe measured chemical characteristics and extracted total DNA from six vinasse batches taken over 1.5 years from a bioethanol and sugar mill in Sao Paulo State. The vinasse microbial assemblage was characterized by low alpha diversity with 5-15 species across the six vinasses. The core genus was Lactobacillus. The top six represented bacterial genera across the samples were Lactobacillus, Megasphaera and Mitsuokella (Phylum Firmicutes, 35-97% of sample reads); Arcobacter and Alcaligenes (Phylum Proteobacteria, 0-40%); Dysgonomonas (Phylum Bacteroidetes, 0-53%); and Bifidobacterium (Phylum Actinobacteria, 0-18%). Potential genes for denitrification but not nitrification were identified in the vinasse metagenomes, with putative nirK and nosZ genes the most represented. Binning resulted in 38 large bins with between 36.0 and 99.3% completeness, and five small mobile element bins. Of the large bins, 53% could be classified at the phylum level as Firmicutes, 15% as Proteobacteria, 13% as unknown phyla, 13% as Bacteroidetes and 6% as Actinobacteria. The large bins spanned a range of potential denitrifiers; moreover, the genetic repertoires of all the large bins included the presence of genes involved in acetate, CO2, ethanol, H2O2, and lactose metabolism; for many of the large bins, genes related to the metabolism of mannitol, xylose, butyric acid, cellulose, sucrose, "3-hydroxy" fatty acids and antibiotic resistance were present based on the annotations. In total, 21 vinasse bacterial draft genomes were submitted to the genome repository.ConclusionsIdentification of the gene repertoires of vinasse bacteria and assemblages supported the idea that organic carbon and nitrogen present in vinasse together with microbiological variation of vinasse might lead to varying patterns of N2O emissions during fertirrigation. Furthermore, we uncovered draft genomes of novel strains of known bioethanol contaminants, as well as draft genomes unknown at the phylum level. This study will aid efforts to improve bioethanol production efficiency and sugarcane agriculture sustainability.
| S-EPMC5822648 | biostudies-literature
Project description:Metatranscriptomic analysis of an acidogenic reactor reveals degradation pathways of sugarcane vinasse-compounds and associated microbiome
| PRJEB59096 | ENA
Project description:[This corrects the article DOI: 10.1186/s13068-018-1036-9.].
| S-EPMC6169111 | biostudies-literature
Project description:Anaerobic Reactor for Vinasse Treatment Metagenome
| PRJNA431586 | ENA
Project description:This study evaluates the benefits of mineral fertilizers replacement for biodigested vinasse. Data from experimental anaerobic digestion (AD) of vinasse were applied to support the analysis. Based on previous experiments, this assessment assumed that vinasse production could reach 2.38 × 107 m3/year generating around 66,585 MWh/year of electric energy from biogas burning in the Administrative Region of Campinas (ARC). This amount of energy could supply more than 103,000 inhabitants and avoid 35,892 tCO2eq/year (from electric energy replacement). The biodigested vinasse might also reduce the total N, P, and K mineral fertilizers demand per hectare of sugarcane crop in 30%, 1%, and 46%, respectively, avoiding additional greenhouse gas emissions of 111,877 tCO2eq/year. There is no biodigested vinasse surplus for a moderate fertigation rate of 100 m3/ha, complying with local environmental laws related to nutrients excess side effects in areas destined to sugarcane crop. Notwithstanding, a Geographic Information System analysis for a small adjacent area to ARC indicated nine different fertigation rates, ranging from 50 to 100 m3/ha. Even though the general analysis for ARC shows high NPK replacement levels, the fertigation practices should be subsidized for robust soil analysis and adequate to safe environmental levels. A management tool can be designed using the results here presented to subsidize investments for AD widespread adoption by the sugarcane industry to catch a reasonable practice from the economic and environmental perspectives.
| S-EPMC7539059 | biostudies-literature
Project description:DNA, RNA and protein were extracted from the culture and subjected to massive parallel sequencing and nano-LC-MS-MS respectively
2010-03-25 | GSE18535 | GEO
Project description:Vinasse is a by-product of sugarcane processing which is often used in fertigation; however, the direct use of vinasse harms the environment and reduces soil productivity due to its physicochemical properties. Anaerobic digestion (AD) offers an alternative to mitigate part of the negative effects. Anaerobic high-rate reactors, which mainly rely on sludge granulation, are mostly used in AD of vinasse wastewater. However, the composition of vinasse such as high concentration of solids and organic matter, high salinity, low pH, and high concentrations of sulfate, affect granule formation, leading to sludge washout. Anaerobic membrane bioreactors (AnMBR) present an alternative for vinasse treatment, eliminating the need for sludge granulation and producing a nutrient-rich effluent with minimal residual organics and no suspended solids. Research on sugarcane vinasse treatment using AnMBRs is limited. Most studies have employed submerged internal membrane modules, highlighting the need for further research with different reactor configurations to enhance process performance. In this study, an AnMBR equipped with an external inside-out crossflow ultrafiltration membrane was compared to an upflow anaerobic sludge blanket (UASB) reactor for the treatment of sugarcane vinasse. At a volumetric organic loading rate of up to 6 g COD. L-1.d-1, the UASB reactor reached 75% ± 7% of COD removal efficiency whereas the AnMBR generated a solids-free effluent and reached 88% ± 2% of COD removal efficiency. Microorganisms such as Clostridia, Bacteroidia, Mesotaga, Syner-01, Dehalococcoidia, Bacteroidia-DMER64, and Methanolinea were found as the most abundant. The results highlight the AnMBR potential as an effective alternative for treating sugarcane vinasse while overcoming the challenges posed by unsatisfactory sludge granulation.
| S-EPMC11600140 | biostudies-literature