Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of M. monacense from extrapulmonary cultures

ABSTRACT: Characterization of M. monacense from extrapulmonary cultures

PROVIDER: PRJEB94028 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genomic analysis and antimicrobial susceptibility profiling of Mycobacterium monacense from extrapulmonary laboratory cultures.

Project description:Genomic analysis and antimicrobial susceptibility profiling of Mycobacterium monacense from extrapulmonary laboratory cultures.

| PRJEB98678 | ENA

Mycolicibacterium monacense

Project description:Mycobacterium monacense overview

| PRJNA174051 | ENA

Mycolicibacterium monacense DSM 44395

Project description:Mycolicibacterium monacense DSM 44395 Genome sequencing

| PRJNA521103 | ENA

Mycolicibacterium monacense DSM 44395

Project description:Mycolicibacterium monacense DSM 44395 Genome sequencing and assembly

| PRJNA173677 | ENA

Establishment and Molecular Characterization of Glioblastoma Primary Cultures

Project description:Glioblastoma, the most frequent and malignant adult brain tumor has been extensively studied; yet no effective treatment exists. To overcome this dismal scenario, it is essential to improve preclinical biological models. This study aimed to establish and molecularly characterize glioblastoma primary cultures. Additionally, it intended to assess the efficacy of molecular-targeted therapies, in the presence of putative targeting cell lines. Five glioblastoma primary cultures were established exhibiting a diversity of chromosomal alterations by aCGH, with gain of chromosome 7 and loss of chromosome 10, 13 and 17p, the most frequent alterations. Mutation profiling by Ion Torrent and Sanger sequencing showed the present of hotspot mutations in TERT (4/5), TP53 (2/5) and RB, BRAF, PTEN and EGFR (1/5). A similar chromosomal and mutation pattern was observed in the primary cultures and matched frozen tumors. The MTS cell viability assay of the p.Gly598Val EGFR mutated cell line, revealed AST1306 as the most potent EGFR inhibitor, when compared with afatinib, erlotinib, or lapatinib. Herein, glioblastoma primary cultures were successfully established and molecularly characterized. Importantly, we showed that AST1306 inhibits EGFR mutated cells, suggesting that primary cultures are suitable in vitro models for glioblastoma biology and preclinical drugs studies.

2016-07-01 | GSE59967 | GEO