ABSTRACT: Metagenome assembly of PRJEB82447 data set (Unveiling diversity and adaptations of the wild tomato microbiome in their center of origin in the Ecuadorian Andes)
Project description:Members of the tomato clade exhibit wide diversity in fruit coloration, growth habit, leaf morphology and mating preferences. However, the mechanisms governing inter-species diversity in fruit coloration are largely unknown. Therefore, a proteomic approach combined with carotenoid profiling and carotenogenic gene expression was used to decipher the diversity in carotenogenesis in green-fruited Solanum habrochaites, orange-fruited S. galapagense, and red-fruited S. pimpinellifolium with S. lycopersicum, cv. Ailsa Craig (tomato).
Project description:The CELL NUMBER REGULATOR/FW2.2-like (CNR/FWL) gene family was named in reference to its founding member, the FW2.2 gene (standing for Fruit Weight QTL on chromosome 2, number 2), which underlies the major quantitative trait locus (QTL) governing fruit size in tomato. The CNR/FWL gene family encompasses hundreds of related sequences in the plant, animal, and fungal reigns (Guo et al., 2010), with a large diversity in protein size ranging from a hundred to several hundred amino acids. In the present study, we aimed at investigating whether SlFWLs proteins play a role in the control of organ growth in tomato and shed light on the critical involvement of SlFWL5 in leaf development. Here we performed a co-immunoprecipitation experiment on protein extract from tomato leaves (leaves 3 and 4 of 6 weeks old plants) from wild-type and 35S::SlFWL5-YFP Ailsa Craig plants in order to identify interacting proteins of SlFWL5.
Project description:The goal of the RNA seq was to investigate the transcriptome changes induced by Pseudmonas syringae pv. tomato J4 in wild-type tomato 'Moneymaker' and transgenic 'Moneymaker' overexpressing the Arabidopsis ELP4 (AtELP4) gene. Results showed that P. syringae pv. tomato J4 induced dramatic transcriptional changes in both the wild-type and transgenic tomato plants. Interestingly, a group of defense genes including PR-5x, Pti5, PR1b1, and CHI3/9/14/17, which are associated with resistance to the hemibiotrophic bacterial pathogen Ralstonia solanacearum, were induced to higher levels in the AtELP4 transgenic tomato than in the wild type at 8 and 24 hr after P. syringae pv. tomato J4 infection. These results indicate that overexpression of AtELP4 in tomato leads to faster and/or stronger induction of some defense genes.
Project description:Tomato plants are submitted to a high diversity of herbivory pests, among them the leafminer Tuta absoluta, considered as one of the most important threat on the tomato worldwide production. In spite of its susceptibility to this pest, a better understanding of the tomato plant response to T. absoluta herbivory will help defining plant resistance traits and enlarging the range of possibilities for an efficient integrated pest management strategy. We analyzed the transcriptomic response in leaves of tomato (cv. Better Bush) submitted to the herbivory of T. absoluta larvae after 5h and 24h.
