Project description:High-throughput sequencing of endogenous small RNAs from the moss Physcomitrella patens. This dataset encompasses microRNAs and other small RNAs of ~20-24 nucleotides expressed in the moss P. patens. SAMPLES UPDATED JULY 9, 2007 TO INCLUDE DATA ON SEQUENCED SMALL RNAS THAT DO NOT MATCH THE P. PATENS GENOME Keywords: High throughput small RNA sequencing
Project description:4plex_physco_2014-05 - ppmax2 response to gr24 - How does the Ppmax2 moss mutant respond to Strigolactone (GR24)? - Two moss genotypes are used: WT and the Ppmax2 mutant. Moss tissues are fragmented, then plated on medium (Petri dish with cellophane disks) and cultivated for 3 weeks. Moss tissues are then transfered for 6 hours on acetone-containing medium (control treatment, for WT and Ppmax2) or GR24 (1 microM, in acetone)-containing medium (for Ppmax2). After 6 hours, the moss tissues are collected, quickly forzen in liquid nitrogen. RNA are isolated using the Quiagen RNeasy Plant mini kit (including a RNase-free DNase treatment on column). Two similar experiments (T1 and T2) have been led.
Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
Project description:Lychee (Litchi chinensis Sonn.) is a popular fruit worldwide. Lychee downy blight, caused by Peronophythora litchii (P. litchii), is one of the major diseases in lychee. In this study, we conducted a P. litchii infection experiment on the lychee leaves of GW and SFZ, and analyzed the mRNA in the treated leaves using transcriptome RNA sequencing technology. Our research will provide a preliminary basis for the prevention and control of lychee downy blight.
Project description:Non coding RNA (ncRNA), such as miRNA, lncRNA and circRNA, has been found to play an important role in the mechanism of plant defense against pathogens. Lychee downy blingt is the main disease of litchi fruit, inflorescence and leaf, which seriously affects the yield of lychee. In this study, we carried out a P.litchii infection experiment on lychee fruits and leaves, and the mRNA and ncRNA in the treated fruits and leaves were analyzed by whole-transcriptome RNA sequencing technology. our work will Provide new insights for the resistance of lychee downy blight .
Project description:This project aimed to discover genes that regulate the transition from 2D to 3D growth in the moss Physcomitrella patens. Mutants were generated that failed to initiate 3D growth. Bulk segregant analysis was conducted to identify the causative genes. This experiment contains four samples - GdGFP, VxmCherry, WT-pool, Mt-pool.
Project description:We constructed three moss aldh5F2, aldh10A1 and aldh21A1 knockouts to obtain complementary information on the importance of each family for the regulation of GABA shunt and levels of metabolites in TCA cycle.
