Project description:The present research investigates a ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) tuber proteome with an aim to unravel its proteome using a high-throughput proteomics technique. Although JA has been historically know to the Native Americans, it was brought back and spread to Europe by the colonists and in the late 19th century early 20th century it began to regain importance including its use for health and as a folk remedy for diabetes. In Japan (referred to as ‘kiku-imo’) its cultivation became popular mostly for health-related benefits such as reducing the blood sugar level. The group of (Genboku Takahashi et al.) has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life, and thus the research progressed to deeply look into the protein components through proteomics as very less is known about the proteome of the tubers, especially in relation to its importance as a functional food in treating diseases health conditions like diabetes. Using three commercially processed JA tuber products we used total protein extraction on the powdered samples in conjunction with label-free quantitate proteomic approach (mass spectrometry) to identify for the first time a comprehensive protein list for the JA tuber. A total of 2967 high‐confidence proteins were identified and categorized into different protein classes through bioinformatics. We have discussed these proteins especially in relation to their association with health and disease regulatory metabolism.
Project description:Endurance exercise has a dramatic impact on the functionality of mitochondria and on the composition of intestinal microbiome, but the mechanisms regulating the crosstalk between these two components are still largely unknown. Here, we sampled 20 elite horses before and after an endurance race and used blood transcriptome, blood metabolome and fecal microbiome to describe the microbiota-mitochondria inter-talk. A subset of mitochondria-related differentially expressed genes involved in pathways such as energy metabolism, oxidative stress and inflammation was discovered and then shown to be associated with butyrate-producing bacteria of the Lachnospiraceae family, especially Eubacterium. The mechanisms involved were not fully understood, but through the action of their metabolites likely acted on PPARδ, the FRX-CREB axis and their downstream targets to delay the onset of hypoglycemia, inflammation and extend running time. Our results also suggested that circulating free fatty acids may act not merely as fuel but drive mitochondrial inflammatory responses triggered by the translocation of gut bacterial polysaccharides following endurance. Targeting the gut-mitochondria axis appears therefore as a potential strategy to enhance athletic performance.
2021-02-09 | GSE163767 | GEO
Project description:Salt stress RNA-seq of Jerusalem artichoke seedlings
| PRJNA793515 | ENA
Project description:Soil fungal communities in th Jerusalem artichoke field