Project description:To examine the difference of the endometrial conditions in the cows between on summer and autumn seasons, gene expression profiles were compared. The expressions of 268 genes were significantly higher in the endometrium collected on summer than those on autumn, whereas 369 genes were lower (P<0.05 or lower). Transcripts of GP2 and NTS were more abundant in the endometrium of summer than those of autumn (P<0.05). In contrast, the mRNA expressions of CDH1 and HSPH1 were lower in the endometrium of summer seasons (P<0.1). Collectively, the different gene expression profiles may contribute to functional differences of endometrium between the summer and autumn seasons, and GP2 and NTS may have a relevance to endometrial deficiency that cause infertility of cows on summer seasons.
Project description:To identify the concurrent effects of multiple environmental factors on gene expression under natural climatic fluctuations in rice, we examined global gene expression patterns in two fields typical of the main modes of rice cultivation, over two seasons, each comprising 15 time-points in three different genotypes
Project description:Two fiber tissues harvested 10 days post anthesis from upland cotton trees grown under the same green house conditions except for different seasons of the year were used for RNA extraction. Small RNA molecules under 30 bases were amplified and isolated from an agarose gel. The purified DNA was used directly for cluster generation and sequencing analysis using the Illumina Genome Analyzer according to the manufacturer's instructions. The 35nt sequence tags from sequencing went through data cleaning first, which included getting rid of the low-quality tags and several kinds of contaminants from the 35nt tags. All clean tag sequences with copy numbers were then summarized into a fasta format file. Fiber samples from different seasons were used for small RNA sequencing and data processing.
Project description:At the regional scale, although environmental factors are known to shape the distributions of belowground communities in terrestrial ecosystems, these environmental factors account for relatively low percentages of the variation in belowground communities. More of this variation might be explained by considering ecosystem stable isotopic values, which can provide insight into environmental conditions. Here, we investigated ecosystem (plant and soil) δ13C and δ15N values and belowground communities (microbes and nematodes) as well as environmental factors (climates, soils, and plants) across the Mongolian Plateau. The regression analyses showed that plant isotopic values were more closely associated with belowground communities than soil isotopic values, while ecosystem δ13C values were more closely associated with the belowground communities than ecosystem δ15N values. We also found isotopic values were more closely associated with nematode communities than microbial communities. Variation partioning analyses indicated that environmental variables together explained 16-45% of total variation in belowground communities. After isotopic variables were added as predictors to the variation partition analyses, the explanation of the variance was improved by14-24% for microbial communities and was improved by 23-44% for nematode communities. These findings indicate that isotopic values could be used to predict the properties of belowground communities at a regional scale.
Project description:Male song sparrows (Melospiza melodia) are territorial year-round; however, neuroendocrine responses to simulated territorial intrusion (STI) differ between breeding (spring) and non-breeding seasons (autumn). In spring, exposure to STI leads to increases in luteinizing hormone and testosterone, but not in autumn. These observations suggest that there are fundamental differences in the mechanisms driving neuroendocrine responses to STI between seasons. Microarrays, spotted with EST cDNA clones of zebra finch, were used to explore gene expression profiles in the hypothalamus after territorial aggression in two different seasons. Free-living territorial male song sparrows were exposed to either conspecific or heterospecific (control) males in an STI in spring and autumn. Behavioral data were recorded, whole hypothalami were collected, and microarray hybridizations were performed. Quantitative PCR was performed for validation. Our results show 262 cDNAs were differentially expressed between spring and autumn in the control birds. There were 173 cDNAs significantly affected by STI in autumn; however, only 67 were significantly affected by STI in spring. There were 88 cDNAs that showed significant interactions in both season and STI. Results suggest that STI drives differential genomic responses in the hypothalamus in the spring vs. autumn. The number of cDNAs differentially expressed in relation to season was greater than in relation to social interactions, suggesting major underlying seasonal effects in the hypothalamus which may determine the differential response upon social interaction. Functional pathway analyses implicated genes that regulate thyroid hormone action and neuroplasticity as targets of this neuroendocrine regulation.
Project description:Different fuctional genes have different expression levels in various tissues. Along with the seasons changing, expression level also changes. One-year expression level for functional genes can be indicated by the four seasonal samples and respective time-course change can also be detected. We use microarry chips to study the expression levels for specific genes and explore new functional genes involved in secondary metabolism
Project description:Different fuctional genes have different expression levels in various tissues. Along with the seasons changing, expression level also changes. One-year expression level for functional genes can be indicated by the four seasonal samples and respective time-course change can also be detected. We use microarry chips to study the expression levels for specific genes and explore new functional genes involved in secondary metabolism Samples from four tissues (bud, root, xylem and phloem) were collected in different seasons in one year. April and May were the months in spring for collecting samples. June and July were the months in summer for collecting samples. September and October were the months in autumn for collecting samples. December was the month for collecting samples in winter. RNA from each sample was extracted for hybridization.
Project description:Two fiber tissues harvested 10 days post anthesis from upland cotton trees grown under the same green house conditions except for different seasons of the year were used for RNA extraction. Small RNA molecules under 30 bases were amplified and isolated from an agarose gel. The purified DNA was used directly for cluster generation and sequencing analysis using the Illumina Genome Analyzer according to the manufacturer's instructions. The 35nt sequence tags from sequencing went through data cleaning first, which included getting rid of the low-quality tags and several kinds of contaminants from the 35nt tags. All clean tag sequences with copy numbers were then summarized into a fasta format file.
Project description:Global gene expression analysis of grapevine cv. Pinot Noir berries during development and ripening. Time-course comparison of samples collected at three developmental stages (stages 33, 34 and 36 according to the modified E-L system, ref: Coombe BG, Aust J Grape Wine Res 1995, 1: 104-110) during three seasons (2003, 2005 and 2006).