Ontology highlight
ABSTRACT:
PROVIDER: PRJEB96786 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Geodia barretti (Geodia barretti) genome assembly, odGeoBarr1
| PRJEB96345 | ENA
Project description:Geodia barretti whole genome de novo sequencing
| PRJEB58046 | ENA
Project description:Metatranscriptomics of the marine sponge Geodia barretti: Tackling phylogeny and function of its microbial community.
| PRJEB2885 | ENA
Project description:Marine sponges (phylum Porifera) are leading organisms for the discovery of bioactive compounds from nature. Their often rich and species-specific microbiota is hypothesised to be producing many of these compounds. Yet, environmental influences on the sponge-associated microbiota and bioactive compound production remain elusive. Here, we investigated the changes of microbiota and metabolomes in sponges along a depth range of 1232 m. Using 16S rRNA gene amplicon sequencing and untargeted metabolomics, we assessed prokaryotic and chemical diversities in three deep-sea sponge species: Geodia barretti, Stryphnus fortis, and Weberella bursa. Both prokaryotic communities and metabolome varied significantly with depth, which we hypothesized to be the effect of different water masses. Up to 35.5% of microbial ASVs (amplicon sequence variants) showed significant changes with depth while phylum-level composition of host microbiome remained unchanged. The metabolome varied with depth, with relative quantities of known bioactive compounds increasing or decreasing strongly. Other metabolites varying with depth were compatible solutes regulating osmolarity of the cells. Correlations between prokaryotic community and the bioactive compounds in G. barretti suggested members of Acidobacteria, Proteobacteria, Chloroflexi, or an unclassified prokaryote as potential producers.
2023-05-12 | MTBLS1388 | MetaboLights