Project description:Factorial Microarray Analysis of Zebra Mussel (Dreissena polymorpha) Adhesion Process under the Impact of Multiple Environmental Factors The expression profiles of the zebra mussel byssus unique genes in our cDNA microarray can be influenced by multiple factors. Three environmental factors plus adhesion status were considered as four main factors in this study.
Project description:Blue mussel larvae were fed, in a first group, a balanced diet of essential fatty acids (EFAs) provided by a cocktail diet (COC) from three algal species. Larvae were cultured in three separate tanks from hatching, 0 day post-fertilization (DPF) until 42 DPF. Treated larvae were fed a deficient diet (Tiso) that contains low levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA), two EFAs necessary for larval development, performance, and survival. The goal is to identify coordinated patterns of gene expression and understand their predictive function in relation to growth and mortality during early developmental stages of the blue mussel Mytilus edulis. In order to understand the mechanisms by which growth and survival drive an organism to the full range of its adaptation, we de novo assembled of the mussel transcriptome during early development using next-generation sequencing (NGS) technology, then designed customized microarrays targeting every developmental stage, which encompass major transitions in tissue organization of the fast-evolved blue mussel
Project description:A zebra mussel byssus cDNA microarray was used to identify the differentially expressed genes between attachment and detachment. Keywords: Gene differential expression
Project description:Transcriptional analysis of the effects of natural environmental variation across the vertical distribution of Mytilus californianus within a single mussel bed Keywords: Environmental Response
Project description:We used a cDNA microarray previously defined for the marine sentinel organism Mytilus galloprovincialis (MytArray1.0) to evaluate the effects of nanomolar doses of combined metal salts (50, 100 and 200 nM mixtures of Cd, Cu and Hg) after 48 hours of mussel exposure. Pointing to the mussel gills, first target of toxic water contaminants and actively proliferating tissue, we found significant dose-related increases of cells with micronuclei and other nuclear abnormalities in the treated mussels, with differences in the bioconcentration of the three metals determined in the mussel pulp by atomic absorption spectrometry. Following gill RNA purification and DNA microarray analysis, individual gene expression profiles revealed some transcriptional changes at the 50 nM dose, and substantial increases of differentially expressed genes at the 100 and 200 nM doses with roughly similar amounts of up- and down-regulated signals. The functional annotation of transcripts with consistent expression trends and significantly altered at least in one dose point disclosed the complexity of the induced cell response.
