Project description:In this field experiment, the agronomic performance of Guinea grass was evaluated to assess the optimum planting spacing and harvesting age in mixed cultivation under irrigation conditions. These factors were arranged according to a 3*3 factorial setting, which was fitted to a randomized complete block design and replicated three times. This trial used three planting spaces (20, 30 and 40 cm) and harvesting ages (60, 90 and 120 days), grown in a mixed cropping system with Silver leaf desmodium using a furrow irrigation. The collected data includes plant height, leaves per plant, leaf length per plant, leaf to stem ratio, number of tillers per plant, number of roots per plant, root length per plant, and dry matter yield. The results of the study showed that age at harvest and planting space had a significant (P<0.001) effect on the morphological characteristics and dry matter yield of Guinea grass. The effect of these management practices was also affected the dry matter, crude protein and crude fiber contents of the grass. The maximum performances (high records) of the morphological characteristics and dry matter yield of Guinea grass were obtained by allocating a planting space of 40 cm and prolonging the harvesting period up to 120 days. However, harvesting at 90 days (11.48% of crude protein) resulted in the optimum nutritional contents. Therefore, in the area with irrigation facilities at midland agroecology, a planting space of 40 cm and a harvesting time of 90 days could be suggested through irrigation application in the study area and similar agro-ecologies.
Project description:Scophthalmus maximus spleen proteome after polyI:C-injection under high or low temperature were compared by iTRAQ(isobaric tags for relative and absolute quantitation).
Project description:Forage grasses are mainly used in animal feed to fatten cattle and dairy herds, and guinea grass (Megathyrsus maximus) is considered one of the most productive of the tropical forage crops that reproduce by seeds. Due to the recent process of domestication, this species has several genomic complexities, such as autotetraploidy and aposporous apomixis. Consequently, approaches that relate phenotypic and genotypic data are incipient. In this context, we built a linkage map with allele dosage and generated novel information of the genetic architecture of traits that are important for the breeding of M. maximus. From a full-sib progeny, a linkage map containing 858 single nucleotide polymorphism (SNP) markers with allele dosage information expected for an autotetraploid was obtained. The high genetic variability of the progeny allowed us to map 10 quantitative trait loci (QTLs) related to agronomic traits, such as regrowth capacity and total dry matter, and 36 QTLs related to nutritional quality, which were distributed among all homology groups (HGs). Various overlapping regions associated with the quantitative traits suggested QTL hotspots. In addition, we were able to map one locus that controls apospory (apo-locus) in HG II. A total of 55 different gene families involved in cellular metabolism and plant growth were identified from markers adjacent to the QTLs and APOSPORY locus using the Panicum virgatum genome as a reference in comparisons with the genomes of Arabidopsis thaliana and Oryza sativa. Our results provide a better understanding of the genetic basis of reproduction by apomixis and traits important for breeding programs that considerably influence animal productivity as well as the quality of meat and milk.